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UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING METHODOLOGY
SSP = PCR-SSP, SSOP = PCR-SSOP, revSSOP= reverse SSOP, LUM = Luminex
SAMPLES 4A1 01 - 05/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab Date Date Method used No. received tested
Source of Primers and Probes Commercial
Own
Kits
Design
NT
Other
Detection method
Comments
NT One Lambda Olerup Invitrogen Multiplex PCR-SSP, KIR primers designed by Daved Senitzer (Sun et al. Tissue Antigens 2004, 64(4):462-8)
NT Fluorescence Gel Gel Gel
Unable to make a return for the current cycle
9 38 48 158 193
NT 11-Feb 11-Feb 12-Feb 12-Feb
NT 18-Feb 09-Mar 23-Feb 26-Feb
NT SSO SSP SSP SSP
NT Yes Yes Yes
201 220 276
10-Feb 05-Feb 10-Feb
11-Feb 22-Feb 11-Feb
SSP SSP SSP
Yes Yes Yes
Olerup Invitrogen Olerup
Gel Do not perform KIR haplotyping Gel Fluorescence, Capillary Electrophoresis (Fragment Analyzer AATI)
SSP
Yes
Olerup
Gel
343
10-Feb
11-Feb
NT
Kit manufacturer
Yes
We can clearly report A/B haplotype only for sample 4A1 03/2016 which is B and B (B+B). For other samples the haplotype is A or B and B (A/B+B).
Page 1 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLE 4A1 01/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos A Pos Pos Pos A Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos DEL Pos Pos Pos DEL Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos NT Pos Pos Pos Pos
Page 2 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLE 4A1 02/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos B Pos Pos Pos B Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Page 3 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLE 4A1 03/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos DEL Pos Pos Pos DEL Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg NT Neg Neg Neg Neg
NT Pos Pos Pos FUL NT Pos Pos Pos Pos
Page 4 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
Haplotype A Haplotype B Comment
A A A A
B B B B B B
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLE 4A1 04/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos B Pos Pos Pos B Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Page 5 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLE 4A1 05/2016 DISPATCHED ON 09TH FEBRUARY 2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos A Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos FUL Pos Pos Pos FUL Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Page 6 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
UK NEQAS FOR H&I PILOT SCHEME – KIR GENOTYPING SAMPLES 4A1 01 - 05/2016 NOT ASSESSED DISPATCHED ON 09TH FEBRUARY 2016 SAMPLE 4A1 01/2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos A Pos Pos Pos A Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos DEL Pos Pos Pos DEL Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos NT Pos Pos Pos Pos
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
SAMPLE 4A1 02/2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos B Pos Pos Pos B Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
SAMPLE 4A1 03/2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos DEL Pos Pos Pos DEL Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg NT Neg Neg Neg Neg
NT Pos Pos Pos FUL NT Pos Pos Pos Pos
Haplotype A Haplotype B Comment
A A A A
B B B B B B
SAMPLE 4A1 04/2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos B Pos Pos Pos B Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
SAMPLE 4A1 05/2016 Lab No.
2DL1
2DL2
2DL3
2DL4
2DL5
3DL1
3DL2
3DL3
3DS1
2DS1
2DS2
2DS3
2DS4
2DS5
2DP1
3DP1
9 38 48 158 193 201 220 276 343
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos A Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos Pos Pos Pos Pos Pos
NT Pos Pos Pos FUL Pos Pos Pos FUL Pos Pos
NT Neg Neg Neg Neg Neg Neg Neg Neg
NT Pos Pos Pos NT Pos Pos Pos Pos
NT Pos Pos Pos DEL NT Pos Pos DEL Pos Pos
Haplotype A Haplotype B Comment
A A A
B B B B
A A
B B
Page 7 of 7 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
