Analytical - ACS Publications - American Chemical Society

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DECEMBER 15, 1993 VOLUME 65 NUMBER 24 CHEMISTRY ANCHAM 65(24) 1055A-1096A/3545-3784(1993) ISSN 0003-2700 Registered in U.S. Patent and Trademark Office; ©Copyright 1993 by the American Chemical Society EDITOR: ROYCE W. MURRAY, University of North Carolina ASSOCIATE EDITORS: Catherine C. Fenselau, University of Maryland Baltimore County, William S. Hancock, Genentech, James W. Jorgenson, University of North Carolina, Robert A. Osteryoung, North Carolina State University, Edward S. Yeung, Iowa State University/Ames Laboratory Editorial Headquarters, research section Department of Chemistry Venable and Kenan Laboratories University of North Carolina Chapel Hill, NC 27599-3290 Phone:919-962-2541 Fax:919-962-2542 E-mail: Murray @ uncvx1.oit.unc.edu Editorial Headquarters, A-page section 1155 Sixteenth St., N.W. Washington, DC 20036 Phone:202-872-4570 Fax: 202-872-4574 E-mail: acx96 @ acs.org Managing Editor: Mary Warner Senior Editor: Louise Voress Associate Editor: Grace K. Lee Assistant Editor: Felicia Wach

REPORT 1068 A On the cover. NMR imaging of materials. As NMR imaging has become a major diagnostic tool in clinical medicine, its use in nonmedical applications has been less publicized. Richard A. Komoroski of the University of Arkansas for Medical Sciences assesses the status and future of the technique and emphasizes the types of information available in NMR images

A/C INTERFACE 1087 A Computer-assisted structure elucidation. Spectral interpretation can be automated by library searching, artificial intelligence and pattern recognition techniques, and spectral simulation. In the second of a two-part series, Wendy A. Warr of Wendy Warr and Associates examines artificial intelligence, pattern recognition, and spectral simulation

Editorial Assistant: Deborah Noble Contributing Editor: Marcia Vogel Head, Graphics and Production: Leroy L. Corcoran Art Director: Alan Kahan Designers: Sarah Chung, Peggy Corrigan Production Editors: Kathleen Savory, Elizabeth Wood Electronic Composition: Wanda R. Gordon Circulation: David Schulbaum Journals Dept, Columbus, Ohio Editorial Office Manager: Mary E. Scanlan Journals Editing Managers: Kathleen E. Duffy, Anne C. O'Melia, Joseph E. Yurvati Associate Editor: Lorraine Gibb Assistant Editor: Brenda S. Wooten Advisory Board: Michelle V. Buchanan, Bruce Chase, M. Bonner Denton, Joseph G. Gordon, David M. Haaland, Joel M. Harris, Timothy D. Harris, Franz Hillenkamp, Kiyokatsu Jinno, Dennis C. Johnson, Richard A. Keller, Philip D. LaFleur, Gary E. Maciel, Géraldine Richmond, Ralph Riggin, Michael Thompson Ex Officio: Joseph L. Glajch

Instrumentation Advisory Panel: Anna Brajter-Toth, Raymond E. Clement, Therese M. Cotton, Norman J. Dovichi, Jack D. Henion, Mary Ellen P. McNally, John W. Olesik, Dallas L. Rabenstein, J. Michael Ramsey Publications Division Director: Robert H. Marks

NEWS 1067 A NMR imaging of food. · An aquifer comes clean. · Internet reference updated. · MRS database MEETINGS 1078 A Pittcon '94 will convene at Chicago's McCormick Place-on-the-Lake Convention Center Feb. 27-March 4. · Conferences. · Short courses and workshops. · Call for papers BOOKS A volume on FT Raman spectroscopy is reviewed

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NEW PRODUCTS AND MANUFACTURERS' LITERATURE

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Head, Special Publications: Anthony Durniak Head, Journals: Charles R. Bertsch BPA International Business Publication Membership Applied for August 1993.

ANALYTICAL CHEMISTRY, VOL. 65, NO. 24, DECEMBER 15, 1993 · 1059 A

ANALYTICAL CHEMISTRY (ISSN 0003-2700) is published semimonthly by the American Chemical Society, 1155 Sixteenth St., N.W., Washington, DC 20036. Second-class postage paid at Washington, DC, and additional mailing offices. Postmaster: Send address changes to ANALYTICAL CHEMISTRY, Member & Subscriber Services, P.O. Box 3337, Columbus, OH 43210. Canadian GST Reg. No. R127571347. Copyright Permission: An individual may make a single reprographic copy of an article in this publication for personal use. Reprographic copying beyond that permitted by Section 107 or 108 of the U.S. Copyright Law is allowed, provided that the appropriate per-copy fee is paid through the Copyright Clearance Center, Inc., 27 Congress St., Salem, MA 01970. For reprint permission, write Copyright Administrator, Publications Division, ACS, 1155 Sixteenth St., N.W., Washington, DC 20036. Registered names and trademarks, etc., used in this publication, even without specific indication thereof, are not to be considered unprotected by law.

Accelerated Articles Characterization of Antisense Binding Properties of Peptide Nucleic Acids by Capillary Gel Electrophoresis 3545 Donald). Rose Study of Zone Broadening in Optically Gated High-Speed Capillary Electrophoresis Alvin W. Moore, Jr., and James W.Jorgenson* Continuous-Flow Immunosensor for Detection of Explosives James P. Whelan, Anne W. Kusterbeck, Gregory A. Wemhoff, Reinhard Bredehorst, and Frances S. Ligler*

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Jet Ring Cell: A Tool for Flow Injection Spectroscopy and Microscopy on a Renewable Solid Support Jaromir Ruzicka*, CyH. Pollema, and Kurt M. Scudder

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Ultrasensitive Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in Capillary Electrophoresis ShumingNie, Rajeev Dadoo, and Richard N. Zare *

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Subscription and Business Information

Articles

1993 subscription rates include air delivery outside the U.S., Canada, and Mexico. Canadian subscriptions are subject to 7% GST.

X-Ray Continuum Discrimination Technique for the Energy Dispersive Analysis of Fine Particles Laura E. Giblin, William H. Blackburn, and David M. Jenkins *

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Noninvasive Monitoring of Bulk Polymerization Using Short-Wavelength Near-Infrared Spectroscopy P. K. Aldridge*,J. J. Kelley, JamesB. Callis, andD. H. Burns

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Flexible Conductimetric Sensor Kohji Mitsubayashi, Kenji Yokoyama, Toshifumi Takeuchi, Eiichi Tamiya, and Isao Karube * Perturbation of the Electrified Interface and the Response of the Thickness-Shear Mode Acoustic Wave Sensor under Conductive Liquid Loading Mengsu Yang and Michael Thompson * Scanning Electrochemical Microscopy. 23. Reaction Localization of Artificially Patterned and Tissue-Bound Enzymes David T. Pierce and Allen J. Bard*

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Scanning Electrochemical Microscopy. 24. Enzyme Ultramicroelectrodes for the Measurement of Hydrogen Peroxide at Surfaces 3605 Benjamin R. Horrocks, David Schmidtke, Adam Heller*, and Allen J. Bard* Simulation of Polysaccharide 13 C Nuclear Magnetic Resonance Spectra Using Regression Analysis and Neural Networks Jon W. Ball and Peter G Jurs *

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Joule-Discharge Heating Studies of Pore Connectivity in Silica Gel: Influence of Pore Diameter, Chemical Modification, and Particle Size E. H. Ellison, S. W. Watte, D. B. Marshall, and J. M. Harris *

"Corresponding author

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ACS membership information: Lorraine Bowlin (202-872-4567) ANALYTICAL CHEMISTRY, VOL. 65, NO. 24, DECEMBER 15, 1993 · 1061 A

analytical® CHEMISTRY

Editorial Information Instructions for authors of AC RESEARCH are published in the January 1 issue, p. 91. Guidelines for the INSTRUMENTATION, REPORT, ANALYTICAL APPROACH, and A/c INTERFACE features are published in the January 1 issue, p. 90. Please consult these instructions and guidelines prior to submitting a manuscript for consideration for publication. Manuscripts for publication in AC RESEARCH (4 copies of text and illustrative material) should be submitted to the Editor at the University of North Carolina address. Please include a signed copyright status form; a copy of this document appears on p. 95 of the January 1 issue. Manuscripts for publication in the A-page section should be submitted to the Washington editorial staff. For individual reprints of AC RESEARCH or A-page articles, please contact the authors directly. Bulk reprints of individual articles are available from ACS. For information, write or call the Distribution office at the ACS Washington address (202-872-4539; fax 202-872-4615). ACS Division of Analytical Chemistry Chair, Janet Osteryoung (919-515-2355) Secretary, Sarah Rutan (804-367-1298) ACS Information Library Services Education Division Meetings Dept. Member Services Employment Services Public Outreach

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Supplementary material is noted in the table of contents with a • It is available as photocopy ($10.00 for up to 3 pages and $1.50 per page for additional pages, plus $2.00 for foreign postage) or as 24X microfiche ($10.00, plus $1.00 for foreign postage). Canadian residents should add 7% GST. See supplementary material notice at end of journal article for number of pages. Orders musf state whether for photocopy or for microfiche and give complete title of article, names of authors, journal, issue date, and page numbers. Prepayment is required and prices are subject to change. Order from Microforms & Back Issues Office at the ACS Washington address. The paper used in this publication meets the minimum requirements of American National Standard for Information Sciences—Permanence of Paper for Printed Library Materials, ANSI Z39.48-1984. Journals Department American Chemical Society 2540 Olentangy River Road P.O. Box 3330 Columbus, OH 43210 614-447-3600, Ext. 3171 TELEX 6842086; Fax 614-447-3745 Member & Subscriber Services American Chemical Society P.O. Box 3337 Columbus, OH 43210 614-447-3776 800-333-9511

Ultrasensitive Heterogeneous Immunoassay Using Photothermal Deflection Spectroscopy Chao-Yi Tu, Takehiko Kitamori, Tsuguo Sawada*, Hiroko Kimura, and Shigetaka Matsuzawa Line Selection and Evaluation of Radio Frequency Glow Discharge Atomic Emission Spectrometry for the Analysis of Copper and Aluminum Alloys Tina R. Harville and R. Kenneth Marcus * Determination of Sulfhydryl Groups in Ovalbumin by High-Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometric Detection Kikuo Takatera * and Tadashi Watanabe *

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Elimination of Frequency Drift from Fourier Transform Ion Cyclotron Resonance Mass Spectra by Digital Quadrature Heterodyning: Ultrahigh Mass Resolving Power for Laser-Desorbed Molecules 3647 Shenheng Guan, Markus C. Wahl, and Alan G. Marshall* Dependence of Ion Intensity in Electrospray Mass Spectrometry on the Concentration of the Analytes in the Electrosprayed Solution Liang Tang and Paul Kebarle *

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Thin Gold Film-Assisted Laser Desorption/Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Biomolecules Markus C. Wahl, Hyun Sik Kim, Troy D. Wood, Shengheng Guan, and Alan G. Marshall*

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Universal Sample Enrichment Technique for Organochlorine Pesticides in Environmental and Biological Samples Using a Redesigned Simultaneous Stream Distillation-Solvent Extraction Apparatus Volker Seidel and Wolfgang Lindner* Direct Chiral Separations by Capillary Electrophoresis Using Capillaries Packed with an o^-Acid Glycoprotein Chiral Stationary Phase Song Li and David K. Lloyd * Liquid Chromatography Based on a Low-Temperature Method of Producing Glassy Carbon Tina M. Engel, Susan V. Olesik *, Matthew R. Callstrom, and Mark Diener

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Correspondence Dependence of Retention and Separation Quality in Planar Chromatography on Properties of the Mobile Phase 3701 David Nurok*, Robert M. Kleyle, Kenneth B. Lipkowitz, Steven S. Myers, and Michelle L. Kearns

Technical Notes Automated Velocity Programming for Increased Detection Zone Residence Times in Capillary Electrophoresis Jason B. Shear, Luis A. Colon, and Richard N. Zare*

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Advertising Office: Centcom Ltd., 1599 Post Road East, P.O. Box 231, Westport, CT 06881 The American Chemical Society and its editors assume no responsibility for the statements and opinions advanced by contributors. Views expressed in the editorials are those of the editors and do not necessarily represent the official position of the American Chemical Society.

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analytical® CHEMISTRY

LabGuide The LabGuide is published as the August 15 issue of ANALYTICAL CHEMISTRY. Begun 38 years ago by the JOURNAL, the annual LabGuide edition is designed to be the ultimate buyers' resource for chemists. Its comprehensive coverage includes 2250 categories of laboratory products and services from more than 1700 companies. The 1994 edition features -40,000 informative listings detailing manufacturers and distributors of each product and service. Each listing includes the company's phone number for fast action; a more complete listing of the company's address and branch offices is located in a separate section at the back of the book. Sections include Chromatography; Spectroscopy; Instruments and Accessories; Equipment and Supplies; Chemicals and Standards; and Research, Analytical, Consulting, Education, and Training Services. Putting together such a comprehensive and useful guide would be impossible without the suggestions and guidance of our advisory board. Current members include Duane Bartak (University of North Iowa), Bob Hoesly (Mettler Toledo), Paul Preidecker (Gilson), K. C. Warawa (K. C. Associates), Anne Cerino (Anne Cerino Marketing Communications), H. M. Kingston (Duquesne University), Tim Heil (Radian), Susan Olesik (Ohio State University), Rodger Stringham (Merck), Bob Wright (Battelle Pacific Northwest Laboratories), and Sherman Hamel (Parr Instruments).

Instrumentation 1988-1991

in Analytical

Acoustic Network Analysis as a Novel Technique for Studying Protein Adsorption and Denaturation at Surfaces MengsuYang,Foun Ling Chung, and Michael Thompson *

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Selective Separation of C60 and C70 Fullerenes on Tetraphenylporphyrin-Silica Gel Stationary Phases C. E. Kibbey, M. R. Savina, B. K. Parseghian, A. H. Francis, and M. E. Meyerhoff *

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Graphite-Coated Metal Mesh Optically Transparent Electrodes Matthias Kummerandjon R. Kirchhoff*

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Large Volume Stacking of Anions in Capillary Electrophoresis Using an Electroosmotic Flow Modifier as a Pump Dean S. Burgi

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Author Index

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Cumulative Author Index

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Keyword Index

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A-Page Index

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Chemistry

This recently published book continues a series begun as Instrumentation in Analytical Chemistry in 1973 by Alan J. Senzel, and continued by Stuart A. Borman in the 1980s as Instrumentation in Analytical Chemistry: Volume 2 and instrumentation in Analytical Chemistry: 1982-86. Like its predecessors, Instrumentation in Analytical Chemistry 1988-1991 is designed to provide readers with updated overviews of analytical techniques and instrumentation drawn from articles originally published in ANALYTICAL CHEMISTRY'S A pages. The volume begins with an introduction by Royce Murray designed to help readers put into perspective the methodological and instrumental developments that have occurred during the period. The book is divided into six topical areas; Robotics, Computers, and Laboratory Data Management; Atomic and Molecular Spectroscopy; Electroanalytical Chemistry and Chemical Sensors; Separations; Mass Spectrometry; and Surface Analysis. Each section begins with a short introduction designed to provide a context for interpreting the articles that follow and to answer questions such as "What are recent changes in this field?" and "Why are these techniques important?". Copies of Instrumentation in Analytical Chemistry 1988-1991 are available In clothbound ($44.95), paperbound ($28.95), and student ($16.95) editions from the ACS Distribution Office, Dept. 390, 1155 16th St., N.W., Washington, DC 20036 (800-ACS-5558).

ANALYTICAL CHEMISTRY, VOL. 65, NO. 24, DECEMBER 15, 1993 · 1065 A