Analytical Chemistry - ACS Publications - American Chemical Society


Analytical Chemistry - ACS Publications - American Chemical Societyhttps://pubs.acs.org/doi/pdfplus/10.1021/ac0124540by...

1 downloads 111 Views 12MB Size

contents

http://pubs.acs.org/ac ISSN 0003-2700

June 1, 2001 / Vol. 73, No. 11

features 312 A

COVER STORY

Microfluidics: Controlling Fluids in Small Places. Most lab-on-a-chip devices are designed to move and direct fluids. Thus, understanding the principles of microfluidics is essential to developing ever more sophisticated miniature analysis systems. Nolan Polson of Thermo Biostar and Mark Hayes of Arizona State University discuss the challenges of microfluidics, list approaches that are being explored, and propose new directions.

Going around the bend. 312 A

320 A

Prefractionation Techniques in Proteome Analysis. Deciphering the human genome was a big job, but proteomic analysis promises to be even more complicated. Not only are there lots of proteins to study, but many are present in only low concentrations. Pier Giorgio Righetti and Annalisa Castagna of the University of Verona (Italy) and Ben Herbert of Proteome Systems (Australia) describe a prefractionation system that helps identify those hard-to-find proteins.

news 301 A

Analytical Currents Biosensing arrays based on RNA. Golden optical sensors. Unsafe air at home. Quantifying chirality. Spin labels for “thrifty” NMR screening. One handy mixing machine! MS for genotyping STRs. No medals for these bacteria.

305 A

Research Profiles ECL for near-field imaging. How electrogenerated chemiluminescence avoids some of the problems of near-field scanning optical microscopy. Multipurpose nanopore sensors. Nature uses them, so why not analytical chemists? Engineering cell-like membrane channels to detect analytes. Putting a fine point on ESI. Creating protein microarrays with electrospray ionization.

308 A

Meeting News Flexible immunoassays on a chip. MS.

Parallel spraying. 307 A

290 A

A N A LY T I C A L C H E M I S T R Y / J U N E 1 , 2 0 0 1

Classifying enzyme inhibitors using

contents

Golden optical sensors. 301 A F

F

F

F

O F

CH2

+ O

F

F PFBHA

NH2

C H

H H

F H

Formaldehyde

C O F

F

H N

C H

Oxime

Unsafe air. 302 A 309 A

Government and Society Microarray standards adopted. Fresenius gets a new identity.

NACLA steps forward.

departments 293 A

Editorial Analytical Chemistry’s Impact. How do you measure the impact of this Journal on the analytical sciences?

295 A

In AC Research

327 A

Product Review DNA Sequencers Rely on CE. The “heroes” of the Human Genome Project can read as many as half a million bases a day.

333 A

AC Educator* Teaching the Essential Principles. Miguel Valcárcel of the University of Córdoba (Spain) argues that learning begins with a sound knowledge of the analytical thought processes and basic principles.

The core of education. 333 A

1C

AC Research Contents

2371–2685

AC Research

2686

Author Index

*Includes supporting information, which is available at http://pubs.acs.org/ac

Proteins, proteins, proteins. 320 A

J U N E 1 , 2 0 0 1 / A N A LY T I C A L C H E M I S T R Y

291 A

Analytical Chemistry (ISSN 0003-2700) is published semimonthly by

the American Chemical Society, 1155 16th St., N.W., Washington, DC 20036. Periodicals postage paid at Washington, DC, and additional mailing offices. Postmaster: Send address changes to Analytical Chemistry, Member & Subscriber Services, P.O. Box 3337, Columbus, OH 43210.

For single issues, back issues and volumes, and microform editions, call the number listed for subscription orders or write the

Microform & Back Issues Office at the Washington address. For quotes and information on bulk reprint orders, contact CJS Reprint Services at 888-257-2134 or 410-819-3991.

Copyright permission: The American Chemical Society holds copy-

Subscription orders may be charged to VISA, MasterCard, or AmEx.

right to all materials published in Analytical Chemistry unless otherwise noted. Reprographic copying beyond that permitted by Section 107 or 108 of the U.S. Copyright Act is allowed for a fee of $20.00 per article copy, paid directly to the Copyright Clearance Center (CCC); 978-7508400. Reference ISSN 0003-2700 in your correspondence with CCC. A record of that code should accompany payment. Direct reprint permission requests to ACS Copyright Office, Publications Division, 1155 16th St., N.W., Washington, DC 20036 (202-872-4368; fax 202-872-6060; [email protected]). Registered names and trademarks, etc., used in this publication, even without specific indication thereof, are not to be considered unprotected by law.

Call toll free 800-333-9511 in the continental U.S. Send mail orders with payment for new and renewal subscriptions to American Chemical Society, P.O. Box 182426, Columbus, OH 43218-2426.

Analytical Chemistry is published in print and electronic format and may be published in other formats, methods, and technologies of distribution, now known or later developed. For all illustrations submitted to and used in Analytical Chemistry, it is understood that they may appear in other formats, methods, and technologies of distribution, including but not limited to reprints of the articles to which they apply. Digital Object Identifier (DOI): The DOI identification system

for digital media has been designed to provide persistent and reliable identification of digital objects. Information on the DOI and its governing body, the International DOI Foundation, can be found at http://www.doi.org. In the print editions, the DOI appears on the bottom of the first page; in Web editions of ACS journals, the DOI appears at the top of the HTML version of an article and at the bottom of the first page in the PDF version. Include the DOI in all document requests. Document delivery: Individual articles appearing in ACS Web editions (which extend back to January 1996) and articles posted electronically as Articles ASAP can be purchased directly on the Web with VISA, MasterCard, or AMEX. Go to http://pubs.acs.org/ac and click on Articles on Command. Purchased articles will be delivered as a PDF file. Articles are also available from Chemical Abstracts Service’s Document Detective Service. For information, contact CAS by phone (800-678-4337), fax (617-447-3648), e-mail ([email protected]), or at http://www.cas.org. 2001 print subscription rates include air delivery outside the U.S., Canada, and Mexico. Canadian subscriptions are subject to 7% GST (GST Reg. No. 127571347). Subscriptions sent to Maryland (5%), District of Columbia (5.75%), and California are subject to applicable sales taxes. Members may share/donate their personal subscriptions with/to libraries and the like but only after 5 years from publication.

Changes of address must include both old and new addresses

with ZIP code and a recent mailing label. Send all address changes to Member & Subscriber Services at the ACS Columbus address. E-mail: [email protected] Please allow 6 weeks for change to become effective. Claims for missing issues will not be allowed if loss was due to failure of notice of change of address to be received in the time specified; if claim is dated (a) North America—more than 90 days beyond issue date, (b) all other foreign—more than 180 days beyond issue date. Hard copy claims are handled at the ACS Columbus address. Instructions for authors of AC Research and guidelines for A-page

features are published in the Jan. 1 issue, p 136, or can be obtained from the Analytical Chemistry home page (http://pubs.acs.org/ac). Please consult these instructions prior to submitting a manuscript for consideration for publication. Manuscripts for publication in AC Research (4 copies of text and

illustrative material) should be submitted to the Editor at the University of North Carolina address. Please include a signed copyright status form; a copy of this document appears on inside back cover of the Jan. 1 issue. Manuscripts for publication in the A-page section should be submitted to the Washington editorial staff. Supporting Information (SI) is noted in the table of contents with

a I . SI is available free of charge via the Internet (http://pubs.acs.org/ac). For information on electronic access, send e-mail to [email protected] or call 202-872-6333. The American Chemical Society and its editors assume no responsibility for the statements and opinions advanced by contributors. Views expressed in the editorials are those of the editors and do not necessarily represent the official position of the American Chemical Society.

Journals Department: American Chemical Society, 2540 Olen-

tangy River Rd., P.O. Box 3330, Columbus, OH 43210 (614-447-3600, ext. 3171; fax 614-447-3745)

Nonmember rates in Japan: Nonmember subscribers in Japan

Member & Subscriber Services: American Chemical Society, P.O. Box 3337, Columbus, OH 43210 (614-447-3776; 800-333-9511)

must enter subscription orders with Maruzen Company Ltd., 3–10. Nihonbashi 2-chome, Chuo-ku, Tokyo 103, Japan. Tel: (03) 272-7211. For multiyear and other rates, call toll free 800-333-9511.

Advertising Management: Centcom, Ltd., 676 East Swedesford Rd., Suite 202, Wayne, PA 19087-1612 (610-964-8061).

2001 SUBSCRIPTION RATES

Print only United States Outside North America

Members

Nonmember personal

Institution

Student

$ 87 222

$ 234 369

$ 996 1131

$ 65 200

Members Web access only Web combined with print Web combined with print outside North America

292 A

Institution (1 Class C subnet)

Institution (Site License)

$ 50 137

$ 1046 1195

$ 1643 1892

272

1330

2027

A N A LY T I C A L C H E M I S T R Y / J U N E 1 , 2 0 0 1

e d i to ri a l

Analytical Chemistry’s Impact F

irst, I am pleased to announce that Analytical Chemistry is now accepting manuscript submissions electronically (http://pubs.acs.org/ac). This is an exciting step by which we hope to better serve the analytical chemistry community. I want to discuss the impact of research papers published in this Journal. Analytical Chemistry, as a general journal, serves the entire range of analytical measurement sciences, selecting papers for publication on the basis of their significance in advancing the fundamentals and applications of analytical measurements. Research articles published in the Journal have historically had a large impact on the course of the discipline; the impact is, in fact, significantly larger than that of any other analytical sciences journal. The associated prestige attracts authors who wish to see their work published in a journal where readers expect to see the best and “hottest” results. Analytical Chemistry greatly values these authors and the readers of their papers. When reading Analytical Chemistry, I find breathtaking the implications of the research articles for the discipline’s future. How is the impact of Analytical Chemistry measured? One way is to consider the many seminal papers—those that open up a long line of further research such as capillary electrophoresis— which have been published in these pages (for example, see Anal. Chem. 2000, 72, 324 A–329 A). There are also quantitative measures. For example, the 901 articles published in 1999 in Analytical Chemistry have been cited in other subsequent papers a total of 44,870 times, a value dwarfing that of other analytical journals. Personal readership of print articles is classically harder to quantify, but a recent library use study (http://www.library. wisc.edu/libraries/Chemistry/cost.htm) showed the “use rate” of ACS journals, including Analytical Chemistry, to be much higher than that of non-ACS journals. In addition, the use of Analytical Chemistry ’s Web edition is readily tracked, and I can report that the number of “views” and downloads has grown steadily since our launch in 1997. In 2000, downloads of Web pages totaled well over one million, or nearly 3,000/day. Finally, the Institute for Scientific Information’s (ISI’s) 1999 “impact factor” for Analytical Chemistry was a high 4.56. The 1999 impact factor is the number of 1999 literature citations to articles (and editorials and letters) published in 1997 and 1998

divided by the sum of the 1997 and 1998 articles. Impact factor is a useful tool for evaluating a journal’s role in providing the most significant research to the community. For every year since 1980—save three—Analytical Chemistry has had the highest impact factor among analytical journals. Impact factors have some eccentricities that their users should appreciate. First, it is possible that a single, not necessarily outstanding, article can dominate the year’s citation, as was the case when the unfortunate 1989 Journal of Electroanalytical Chemistry paper on “cold fusion” garnered an impact factor of 12.2. Second, review journals and “advances” book series should not be compared with research journals, because authors often cite review articles as an efficient way of referencing a large body of older literature. Review journal impact factors tend to be large; for example, Electroanalytical Chemistry (an advances series) had a 6.75 impact factor in 1999 based on a ratio of 735 citations to 3 published articles. When a review publication such as Electroanalytical Chemistry is compared with research journals, it can assume an apparent leadership role, as was the case in ISI’s analytical journal lists for 1998 and 1999. Last, extremely selective research journals such as Science and Nature, which consistently publish a small number of papers, can attain large impact factors; whereas a journal that publishes an increasing number of papers year after year runs the risk of diluting its highly cited ones with those that are “merely” excellent. Interestingly, while Analytical Chemistry published over 35% more papers in 1999 compared with 1993, its impact factor increased from 4.08 to 4.56 during that period. Because growth in published papers and an increase in impact factor tend to run in opposite directions, the increase in the Journal’s impact factor is significant. Analytical Chemistry has a proud heritage of leadership in the analytical discipline, which is maintained by the mutually reinforcing high standards of its authors and reviewers. These scholars are the most valued and significant aspects of this Journal.

J U N E 1 , 2 0 0 1 / A N A LY T I C A L C H E M I S T R Y

293 A

EDITOR Royce W. Murray University of North Carolina

ASSOCIATE EDITORS Daniel W. Armstrong

Reinhard Niessner

Iowa State University/Ames Laboratory

Technische Universität München (Germany)

Catherine C. Fenselau

Robert A. Osteryoung

University of Maryland

North Carolina State University

William S. Hancock

Edward S. Yeung

ThermoFinnigan

Iowa State University/Ames Laboratory

EDITORIAL HEADQUARTERS Research section Department of Chemistry Venable and Kenan Laboratories University of North Carolina Chapel Hill, NC 27599-3290 Phone: 919-962-2541; Fax: 919-962-2542; E-mail: [email protected]

A-page section 1155 16th St., N.W., Washington, DC 20036 Phone: 202-872-4570; TDD: 202-872-6076 Fax: 202-872-4574; E-mail: [email protected] Managing Editor: Alan R. Newman Associate Editors: Felicia Wach, Elizabeth Zubritsky Assistant Editors: Judith Handley, Cheryl M. Harris Staff Editors: Rachel Petkewich, Wilder Damian Smith Web Editor: Christine Brennan Web Assistants: Alex Kim, Elizabeth Rozanskas Contributing Editors: Laura Ruth, James Smith, Marcia Vogel, Thomas J. Wenzel Manager, Copyediting: Elizabeth Wood Production Editor: Doug Roemer Creative Director, Publishing & Creative Services: Julie Farrar Art Director: Sean Kennedy Manager, Production & Imaging: Vincent L. Parker Production/Prepress Specialist: Yang H. Ku Journals Associate Editor: Lorraine Gibb Journals Editing Manager: Debora A. Bittaker Journals Production Associate: Patricia A. Saggio Journals Staff Editor: Priscilla J. Petzinger

Editorial Advisory Board

Isiah Warner

Robert Dunn

Louisiana State University

University of Kansas

Luc Bousse

William B. Whitten

John Fetzer

Caliper Technologies

Oak Ridge National Laboratory

Chevron Research and Technology

Robert M. Corn

R. Mark Wightman

Klaus-Dieter Franz

University of Wisconsin–Madison

University of North Carolina

Merck KGaA (Germany)

Hubert Girault

John Frenz Genentech, Inc.

Ex-Officio Member

Totaro Imasaka

Bruce Chase

Ecole Polytechnique Federale de Lausanne (Switzerland)

Kyushu University (Japan)

DuPont

Niels Heegaard

Barbara Larsen

Statens Serum Institut (Denmark)

Ira Levin

A-page Advisory Panel

National Institutes of Health

Michael Angel

Parke-Davis

Viorica Lopez-Avila

University of South Carolina

J. David Pinkston

Midwest Research Institute

Edgar Arriaga

Procter & Gamble

Victoria McGuffin

University of Minnesota

Kimberly Prather

Michigan State University

Alain Berthod

University of California–Riverside

Klaus H. Mosbach

Carol Robinson

University of Lund (Sweden)

National Center for Scientific Research (France)

Janusz Pawliszyn

Tibor Braun

Zbigniew Stojek

University of Waterloo (Canada)

Eötvös University (Hungary)

University of Warsaw (Poland)

Antonio J. Ricco

Sylvia Daunert

Karen Wahl

ACLARA Biosciences

University of Kentucky

Richard Sacks

Dermont Diamond

Pacific Northwest National Laboratory

University of Michigan

Dublin City University (Ireland)

Douglas Westerlund

Peter Schoenmakers

Marta E. Diaz-Garcia

Uppsala University (Sweden)

University of Amsterdam/Shell Research and Technology Center (The Netherlands)

Universidad de Oviedo (Spain)

Renato Zenobi

Francesco Dondi

Swiss Federal Institute of Technology Zurich

DuPont

University of Ferrara (Italy)

Rachel Loo

Oxford University (U.K.)

Publications Division Director: Robert D. Bovenschulte Director, Publishing Operations: Mary E. Scanlan Director, Special Publications: Mary Warner General Manager, Publishing & Creative Services: Bill Succolosky Circulation Manager: Scott Nathan