LABINDUSTRIES

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NEW BOOKS

by this reviewer, which could possibly lead to some confusion, is in Chapter 3, where the low voltage technique is said to be useful in the analysis of saturated hydrocarbons. This should be cor­ rected to "unsaturated" in future edi­ tions. References are plentiful (1499), listed by chapters, with some inevitable overlap. In conclusion, this book provides the attentive reader with a broad, overall view of the principles and applications of mass spectrometry. Apart from the minor limitations mentioned above, it is a fundamental addition to the grow­ ing literature on the subject. It should be rightfully considered helpful to all the workers in the field and indispens­ able to those interested in the theory and construction of mass spectrometers. Methods in Enzymology.

Vol. VI.

Sidney P. Colowick, Nathan O. Kap­ lan, editors, xviii + 105Jt pages. Academic, Press. Inc.. Ill Fifth Ave., New York 3, Ν. Υ. 1963. $28.

WATER DETERMINATIONS N o False End Points Possible With L/I's Aquametry Apparatus Here's the first aquametry apparatus to combine high precision, easy operation and low cost in one compact unit. Karl Fischer end points are prec sely determined on L/I's self-powered colorcoded electrode indicator. (A twolambda excess of K-F reagent in a 100 ml methanol reaction is detectable.) L/I's apparatus is odorless and mois­ ture proof. Uses original K-F reagent bottle as reservoir. Includes 1 ml "piggy­ back" buret for high accuracy over wide range without changing burets. These and other features make K-F water deter­ minations the simplest, most precise yet. Price: $225. Delivery stock, satisfac­ tion guaranteed. For detailed data, write to

LABINDUSTRIES 1 7 4 0 University A v e n u e Berkeley 3, California Phone TH 3 - 0 2 2 0 Circle No. 52 on Readers' Service Card

76 A

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ANALYTICAL CHEMISTRY

This \-olume completes the planned two-volume supplement to the original four-volume treatise on "Methods in Enzymology." A cumulative index to all six volumes is in preparation. The first part of this volume is con­ cerned with the preparation and assay of enzymes and includes sections on the enzymes of nucleic acid metabolism, respiratory enzymes, enzymes of phos­ phate metabolism, and the enzymes of coenzyme and vitamin metabolism. The second part deals with the prep­ aration and assay of substrates and in­ cludes sections on carbohydrates, lipids and steroids ; proteins and derivatives ; and nucleic acids, coenzymes, and de­ rivatives. The last section contains chapters on special techniques and includes chro­ matographic, diffusion, sedimentation, radiochemical, histochemical, NMR, EPR, infrared, optical rotatory disper­ sion, and electrophoresis methods. Manometric

Techniques.

.',t h

Ed.

W. W. Umbreit, R. H. Burns, J. F. Stauffer. in + 305 pages. Burgess Publishing Co., 426 South Sixth St., Minneapolis 15, Minn. 196.',. $6.50. The fourth edition of this manual de­ scribing methods applicable to the study of tissue metabolism reflects the changes that have occurred by the enormous expansion in metabolic stud­ ies. This edition, like the first, is in­ tended for the beginning graduate stu­ dent and attempts to present the best methods suitable for research and to discuss these techniques in clear lan­ guage.

After an introduction to the Warburg constant volume respirometer, carbon dioxide and bicarbonate, and methods of measurement of carbon dioxide; the calibration of respirometers and gen­ eral techniques applicable to manom­ etry are discussed. Chapter 6 intro­ duces the differential respirometer, while Chapter 7 discusses some methods requiring specialized apparatus in addi­ tion to the respirometer. Chapters 8, 9, and 10 discuss methods for the prepara­ tion and study of tissues and enzymes and for the isolation of particulate com­ ponents. Chapter 10 gives some mano­ metric and chemical methods for the estimation of metabolites and enzyme systems. Chapter 11 and 12 describe spectrophotometric and chromato­ graphic systems, respectively. The last chapter, concerned with the prepara­ tion in the laboratory of important in­ termediates and metabolites, is fol­ lowed by 15 pages of references listed alphabetically according to authors. Techniques in Photomicrography.

E.

B. Brian. A. R. Ten Gate, ix +1/+7 pages. D. Van Nostrand Co., Inc., 120 Alexander St., Princeton, N. J. 1963. $6.75. After introductory chapters on the principles and applications of photo­ micrography and a discussion of equip­ ment and materials, some 70 tech­ niques are given which should enable the inexperienced worker to become familiar with the practice of most photomicrographic procedures. The book contains illustrations of equip­ ment and techniques and step-by-step instructions are given. Analytical Chemistry. Vof. 3 , Parts 5-7. Carl E. Crouthamel, Editor, vii + 102 pages. Macmillan Co., 60 Fifth. Ave., New York 11, Ν. Υ. 1963. Paperbound. $3.50. Part 5, Chemical Monitoring of Heavy Water Moderated Research Re­ actors by L. E. Smythe, describes com­ missioning test and routine chemical monitoring of reactors. It then deals with some special chemical problems in­ cluding corrosion in heavy water, degra­ dation of heavy water, radiation dam­ age to ion exchange resins, fuel element and experimental assembly failure, and the microbiology of heavy water. This part contains 122 references. Part 6, 44 pages by L. E. Smythe and Τ. ΛΙ. Florence, contains a detailed consideration of the recent advances in the analytical chemistry of beryllium. Separation methods, precipitation, mer­ cury cathode electrolysis, solvent ex­ traction, ion exchange, and paper chro-