Klett · •
Introduction to Paper Electrophoresis and Related Methods. Michael Lederer. xii + 191 pages. Elsevier Publishing Co., New York, Ν. Υ., 1955. $7.75. Reviewed by GUNTER ZWEIG, C. F.
Kettering Foundation, YellowSprings, Ohio.
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Designed for the rapid and accurate determina tion of thiamin, riboflavin, and other substances which fluoresce in solution. The sensitivity and stability are such t h a t it has been found particularly useful in determining very small amounts of these substances.
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The field of paper electrophoresis in the past 10 years has attracted a great number of research workers, as attested by the growing number of publications in this field. Besides a number of review articles, only a couple of books have been published dealing exclusively with paper electrophoresis. The re viewer is a co-author with E. L. Durrum and R. J. Block of "Paper Chromatog raphy and Paper Electrophoresis." Michael Lederer, who with E. Led erer wrote a monumental book on chromatography (about 3000 refer ences), has now ventured into the field of paper electrophoresis. This book is very attractive, with a beautiful cover, many excellent photographs and dia grams, and is printed on fine, glossy stock. The type is large and easy to read. The author states in the preface that he has written a short book without including the entire literature on the subject. In this he has succeeded. He has succeeded too well, because the book is a little lacking in detailed experimental procedures, and the reader may have to refer to the original article or buy a commercial apparatus (Spinco is not mentioned). According to the author, paper electrophoresis is to be recommended "where no other separation has been successful." The chapters on organic acids, carbohydrates, and nitrogen com pounds should be viewed in that light, because paper or column chromatog raphy seems to offer a superior resolu tion. The introduction is well written and documented. Lederer cites the work of Lodge (1886) for measurement of ionic velocity in a jelly and of Kendall (1923) for electrophoresis in agar. Lederer discusses the sometimes confus ing semantics of paper electrophoresis; he seems to prefer this term instead of ionophoresis, electrochromatography, etc. The theoretical discussion, including mathematical treatment of mobility, is adequate for one who is not a physical chemist. Two chapters deal with tech niques in paper electrophoresis and con tinuous paper electrophoresis. Figure 18 fails to give a legend. In subsequent chapters, specific appli cations of paper electrophoresis are given, like alkaloids, antibiotics, steroids, and dyes. Among the more detailed ANALYTICAL
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