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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A03/2016 DESPATCHED ON 05TH APRIL 2016 CONSENSUS HLA TYPE: HLA-A1, A3; B8, B35; Cw4, Cw7; DR1, DR17; DQ2, DQ5
9 11 20 28 41 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292
A YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
294 297 299 315 323
YES YES YES YES YES
YES YES YES YES YES
Lab. No.
DQ YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES
YES YES
YES
YES YES YES YES YES
A 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NT 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
A 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 NT 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3
B 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 NT 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8
B 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 NT 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35
1 1 1 1 1
3 3 3 3 3
8 8 8 8 8
35 35 35 35 35
C 4 4 4
HLAC 7 7 7
4
7
4
7
4
7
4
7
4 4
7 7
4
7
4 4
7 7
4
7
DR 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
DR 17 17(3) 17 17 17 17 17 17 17 17 17 17 17(3) 17 17(3) 17
DQ 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
DQ 5 5(1) 5 5 5 5 5 5 5 5 5 5 5(1) 5 5(1) 5
1 1 1 NT 1 1 1
17 17 17 NT 17 17 17
2 2 2 NT 2 2 2
5 5 5 NT 5 5 5
1
17
2
5
1
17
2
5
1
17
2
5
1 1
17 17
2 2
5 5
1
17
2
5
1 1
17 17
2
5
Viability % Date received Date tested U T B Comments 06-Apr 07-Apr 95 95 06-Apr 07-Apr 90 90 90 06-Apr 06-Apr 98 97 06-Apr 06-Apr 06-Apr 12-Apr 06-Apr 07-Apr 99 95 06-Apr 06-Apr 90 85 06-Apr 07-Apr 90 90 06-Apr 07-Apr 100 100 06-Apr 07-Apr 95 95 06-Apr 06-Apr 100 100 90 07-Apr 07-Apr 90 90 08-Apr 11-Apr 80 80 60 07-Apr 08-Apr 06-Apr 15-Apr 100 100 06-Apr 07-Apr 06-Apr 07-Apr 99 50 06-Apr 07-Apr 95 90 07-Apr 07-Apr 90 80 Samples delivered late 06-Apr 06-Apr 99 99 06-Apr 06-Apr 99 99 06-Apr 07-Apr 100 06-Apr 07-Apr >80 06-Apr 07-Apr 95 06-Apr 07-Apr 100 06-Apr 06-Apr 90 06-Apr 98 95 06-Apr 06-Apr 06-Apr 100 04-Apr 04-Apr 98 06-Apr 06-Apr 95 90 06-Apr 08-Apr 100 06-Apr 07-Apr 99 85 06-Apr 07-Apr 100 07-Apr 07-Apr 98 98 07-Apr 07-Apr 85 70 B locus is most likely heterozygous. Probably B71 which was cannot assert with serological typing 06-Apr 06-Apr 06-Apr 06-Apr 07-Apr
07-Apr 07-Apr 06-Apr 07-Apr 08-Apr
100 100 90 99
99 >80 >80
85
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING SCHEME 1A - HLA PHENTOYPING - METHODOLOGY 2016 Lab. No. 9 11 20 28 41 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
Source of HLA Class I Typing trays One Lambda One Lambda Own Tray Innotrain, One Lambda MC Diagnostics One Lambda Innotrain, One Lambda One Lambda One Lambda Innotrain One Lambda One Lambda One Lambda One Lambda One Lambda Biorad, Immucor Biotest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda One Lambda Biotest, Innotrian One Lambda Biorad, Innotrain Formedic, One Lambda One Lambda One Lambda Biorad, BAG One Lambda One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda One Lambda Innotrain, One Lambda MC Diagnostics One Lambda Innotrain, One Lambda One Lambda One Lambda Immucor One Lambda One Lambda One Lambda One Lambda Biorad One Lambda Invitrogen One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda
Suplemental DNA typing performed PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSO, Luminex PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSO RT-PCR-SSP PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSP PCR-SSP/SSO
One Lambda
PCR-SSO PCR-SSO
One Lambda
PCR-SSP
Formedic, One Lambda One Lambda One Lambda
PCR-SSP PCR-SSO
Biorad, BAG One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSO
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 2 of 2
