Scheme 1A - 2017-05

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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A05/2017 DESPATCHED ON 23rd MAY 2017 CONSENSUS HLA TYPE: HLA-A1, A30; B8, B13; Cw6, Cw7; DR7, DR17; DQ2, DQAssessment HLAB Cw DR DQ

HLAA

A

B

B

YES YES

1

30(19)

8

13

20 YES YES YES YES YES

1

30

8

13

28 YES YES

YES YES

1

YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES

1 1 1 1 1 1 1

Lab No.

A

11 YES YES

54 58 62 101 119 120 123

YES YES YES YES YES YES YES

30 30 30 30 30 30 30 30 30(19)

8 8 8 8 8 8 3 8

13 13 13 13 13 13 13 13

1

139 142 145 149 154 159 163 181 186 193 194 206

1 1 1 1 1 1 1 1 1 1 1 1

30 30(19) 30 30 30 30 30 30 30 30 30 30

8 8 8 8 8 8 8 8 8 8 8 8

13 13 13 13 13 13 13 13 13 13 13 13

NT

NT

NT

NT

1 1 1 1 1 1 1

30 30 30 30 30 30 30

8 8 8 8 8 8 8

13 13 13 13 13 13 13

YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES

223 YES YES 225 227 238 245 262 268 279

YES YES YES YES YES YES YES

YES YES YES YES YES YES YES YES YES YES YES YES

6

C

DR

DR

DQ

7

17(3)

2

7 6

7

6

7

6

7 7

6

6 6

7 7

7 7 7 7 7 7 7 7

17 17 17 17 17 17 17 17 17(3)

2 2 2 2 2 2 2 2

17 17(3) 17

2 2 2

7 7 7 7 7 7

17 17 17 17 17 17

2 2 2 2 2 2

Viability % T B

Date received

Date tested

24-May

25-May

24-May

24-May

2

24-May

24-May

ND

24-May 25-May 24-May 24-May 25-May

25-May 25-May 24-May 24-May 25-May

100 95

26-May 26-May

30-May 30-May

95

95

26-May 24-May 24-May 24-May 24-May 24-May 24-May 24-May 24-May 24-May 24-May 26-May

26-May 26-May 02-Jun 25-May 24-May 24-May 24-May 24-May 24-May 24-May 24-May 26-May

75

75

75

100

80

99 99 90

99 99 90 100 Class I HLA typing is performed on PBL 99 98

2

2 2

U

90

95 Class II serological testing was not performed on this sample due to the ongoing issues with class II dynabeads. Please note supplementary DNA typing was performed and the class II type for sample 1A05 2017 was DR17, DR7; DQ2 - using PCR-SSP.

80

5 0

Comments

95 90 20 100

100 99

DNA typing required to all loci as viability of cells insufficient for serological typing. 80 ND = no second antigen detected 90 75 T cell count was very low 100 90 5

HLA-C, HLA-DR, DQ results are direvied from DNA typing results

>80 Samples not tested due to late arrival on Friday afternoon, after lab weekend closure

6

7

17

2

7

17

2 2

7

YES YES

1

30

8

13

7

17

289 YES YES

YES

1

30

8

13

7

17

292 294 299 315 323

YES YES YES YES YES YES YES

1 1 1 1 1

30 30 30 30 30

8 8 8 8 8

13 13 13 13 13

7 7 7 7

17 17 17 17

YES YES YES YES YES

2

7 7 7

284 YES YES

YES YES YES YES YES

DQ

7

13

133 YES YES YES YES YES YES YES YES YES YES YES YES YES YES

8

C

6

7

2 2 2

2 2

26-May 24-May 24-May 24-May 24-May 24-May 24-May

29-May 24-May 26-May 24-May 24-May 24-May 26-May

24-May

24-May

25-May

25-May

26-May 24-May 25-May 26-May 29-May

26-May 24-May 25-May 26-May 29-May

95 98 98 100 100 98

90

98 Very low viability B cells % because of problem with class II dynabeads (One Lambda)

98

40

98

98 Class I Lot 5 OneLAmbda TM144 -------- Class II Lot 10 MDR172

60

100 80 Delivery delay 80

95 90 80 70

80

UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I

NEQ-114 Issue 2 Effective Date 10/10/16 P. 1 of 2

UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A05/2017 DESPATCHED ON 23rd MAY 2017 CONSENSUS HLA TYPE: HLA-A1, A30; B8, B13; Cw6, Cw7; DR7, DR17; DQ2, DQ-

Lab No. 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323

Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSO

Source of HLA Class I Typing trays One Lambda In-house Innotrain, One Lambda

Source of HLA Class II Typing trays One Lambda One Lambda Innotrain, One Lambda

PCR-SSO

Innotrain, One Lambda

One Lambda

Luminex PCR-SSP

Immucor, One Lambda One Lambda One Lambda

Luminex PCR-SSO PCR-SSOP PCR-SSP PCR-SSP, SSO PCR-SSP

Innotrain One Lambda One Lambda Innotrain One Lambda One Lambda Innotrain, Lifecodes Biotest One Lambda

PCR-SSO Luminex PCR-SSP, SSO PCR-SSP

One Lambda One Lambda One Lambda One Lambda

One Lambda One Lambda One Lambda One Lambda

PCR-SSP PCR-SSP PCR-SSP PCR-SSP, Luminex PCR-SSO PCR-SSP

One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda

PCR-SSO

One Lambda

One Lambda

PCR-SSP PCR-SSP

BAG One Lambda One Lambda

BAG One Lambda

Comments

One Lambda One Lambda Biorad One Lambda

One Lambda

UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I

NEQ-114 Issue 2 Effective Date 10/10/16 P. 2 of 2