Scheme 1A

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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A06/2014 DESPATCHED ON 3RD JUNE CONSENSUS HLA TYPE: HLA-A3, A25; B7, B62; Cw7, -; DR103, DR15; DQ6, DQ7 Assessment Lab No. HLA-A HLA-B HLA-Cw HLA-DR HLA-DQ 9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 297 299

YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES

YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES

YES

YES

YES

YES YES

YES YES YES YES YES YES YES YES YES

YES YES YES

YES YES YES YES YES YES YES

YES YES YES YES YES YES YES

YES YES YES YES

YES YES YES YES

YES YES

YES YES

YES

YES

YES YES

YES YES

YES

YES

YES YES YES YES YES

YES YES YES YES

HLA-A

A

B

B

C

3 3 3 3 3 3 3 3 3 NT 3 3 3 3 3 3 3 3 3 3 3 3 NT 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3

25 25 (10) 25 25 25 25 25 25 25 NT 25 25 25 25 25 25 25 25 25 25 25 25 NT 25 25 25 25 (10) 25 (10) 25 (10) 25 25 25 25 25 25 25 25 25 25 25

7 7 7 7 7 7 7 7 7 NT 7 7 7 7 7 7 7 7 7 7 7 7 NT 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7

62 62 (15) 62 62 62 62 62 62 62 NT 62 62 62 62 (15) 62 62 62 62 62 62 62 62 NT 62 62 62 62 (15) 62 (15) 62 (15) 62 62 62 62 62 62 62 62 62 62 62

7 NT 7 NT NT NT NT 7 NT NT 7 NT NT 7 NT NT 7 7 NT NT NT NT NT NT NT NT NT NT NT NT NT NT NT NT 7 NT 7 7 NT NT

C

NT NT NT NT NT NT NT NT NT NT NT

NT NT NT NT NT NT NT NT NT NT NT NT NT NT NT NT NT

NT NT

PLEASE NOTE: NEXT DISTRIBUTION IS ON 15TH JULY 2014 © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

DR

DR

DQ

DQ

Date received

Date tested

103 103 103 103 103 103 103 103 103 NT 103 103 103 103 103 103 103 NT 103 103 103 103 NT 103 103 NT NT NT NT NT 103 NT 103 103 NT 103 NT 103 NT 103

15 15 (2) 15 15 15 15 15 15 15 NT 15 15 15 15 15 15 15 NT 15 15 15 15 NT 15 15 NT NT NT NT NT 15 NT 15 15 NT 15 NT 15 NT 15

6 6 (1) 6 6 6 6 6 6 6 NT 6 6 6 6 6 6 6 NT 6 6 6 6 NT 6 6 NT NT NT NT NT 6 NT 6 6 NT 6 NT 6 NT NT

7 7 (3) 7 7 7 7 7 7 7 NT 7 7 7 7 (3) 7 7 7 NT 7 7 7 7 NT 7 7 NT NT NT NT NT 7 NT 7 7 NT 7 NT 7 NT NT

04-Jun 04-Jun 04-Jun 05-Jun 04-Jun 04-Jun 04-Jun 04-Jun 05-Jun

05-Jun 05-Jun 05-Jun 05-Jun 04 - 10-Jun 09-Jun 05-Jun 04-Jun 05-Jun

04-Jun 04-Jun 04-Jun 06-Jun 05-Jun 04-Jun 04-Jun 05-Jun 04-Jun 03-Jun 04-Jun 04-Jun

05-Jun 05-Jun 05-Jun 06-Jun 06-Jun 05-Jun 05-Jun, 13-Jun 06-Jun 04-Jun 04-Jun 04-Jun 05-Jun

04-Jun 04-Jun 04-Jun 10-Jun 05-Jun 04-Jun 05-Jun 04-Jun 04-Jun 04-Jun 03-Jun 05-Jun 04-Jun 06-Jun 06-Jun 04-Jun 05-Jun

04-Jun 04-Jun 05-Jun 10-Jun 05-Jun 04-Jun 05-Jun 05-Jun 04-Jun 04-Jun 03-Jun 05-Jun 05-Jun 06-Jun 06-Jun 05-Jun 05-Jun

UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A06/2014 DESPATCHED ON 3RD JUNE CONSENSUS HLA TYPE: HLA-

Lab No. 9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 297 299

Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP

U

Viability % T B 95

90

98 99 99

90 99 99

95 95 80

95 90 50

Testing failures

90

Luminex

Tray Manufacturer

Comments

One Lambda One Lambda Own trays, One Lambda (DR) Innotrain Innotrain, One Lambda One Lambda One Lambda Innotrain, One Lambda NT as sample not received.

PCR-SSO SBT

95 100

PCR-SSO PCR-SSO

93

PCR-SSP/SSO

PCR-SSP PCR-SSP PCR-SSP PCR-SSO

98

PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSO PCR-SSP PCR-SSO PCR-SSO

90

93 99

99

100

10 B-cells viability too low for typing

95 90 90 100

85 90 80 100

100 100

98 98

90 98 95

90 95 100

80

95 96 100 90 100 98

PCR-SSO

PCR-SSO PCR-SSP

95 100

80 100

96 90 98 80

BioRad, One Lambda Ingen (One Lambda) One Lambda Innotrain One Lambda BioRad BioRad, Innotrain Biotest One Lambda One Lambda

One Lambda One Lambda One Lambda BioRad BioRad One Lambda Biotest One Lambda One Lambda One Lambda One Lambda BioRad One Lambda One Lambda Ingen BioRad

© Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I