Scheme 1B - 2015-01-02

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UK NEQAS for H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B01&02/2015 DESPATCHED ON 27TH JANUARY 2015 Donor HLA type Donor HLA type

1B01/2015 1B02/2015

HLA-A2,-; B50, B62; Cw6, Cw9; DR4, DR7; DQ2, DQ8 HLA-A68, -; B44, B60; Cw5, Cw10; DR13, DR17; DQ2, DQ6

Summary of Results Total tested Positive Negative NT/Equivocal % Positive % Negative

108 1 107 1 0.9% 99.1%

108 1 107 1 0.9% 99.1%

Consensus

Negative

Negative

Lab No

Result 1B01/2015

Result 1B02/2015

Date received

Date tested

Method

1 3 4 7 8 9 10 11 12 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 31 32 34 35 37 39 40 41 42 45 46 48 54 55 57 58 62 66 67 68 78 83 84 85 86 90 93 96 101 103 104 105 106 107 108 110 111 112 113 115 126 127 128 129 130 137 142 153 154 156 174 176 185 198 199 209 219 223 225 232 234 253 255 256 257 269 270 273 276 278 279 285 288 295 298 300 305 306 307 310 317 319 324 325 326

Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative

Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative

02-Feb 29-Jan 28-Jan 28-Jan 29-Jan 28-Jan 28-Jan 26-Jan 04-Feb 28-Jan 28-Jan 28-Jan 29-Jan 28-Jan 28-Jan 29-Jan 28-Jan 29-Jan 28-Jan 29-Jan 28-Jan 29-Jan 28-Jan 28-Jan 28-Jan 29-Jan 28-Jan 28-Jan 28-Jan 29-Jan 28-Jan 28-Jan 29-Jan 28-Jan

19-Feb 29-Jan 10-Feb 30-Jan 29-Jan 29-Jan 30-Jan 04-Feb 09-Feb 30-Jan 29-Jan 03-Feb 04-Feb 29-Jan 29-Jan 29-Jan 28-Jan 30-Jan 29-Jan 02-Feb 29-Jan 29-Jan 03-Feb 30-Jan 28-Jan 29-Jan

Melting curve analysis Flow Cytometry PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO/Flow Cytometry PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP/Melting curve analysis PCR-SSP PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSO PCR-SSP/SSO Flow Cytometry Flow Cytometry

28-Jan - 12-Feb 05-Feb 03-Feb 29-Jan 28-Jan 03-Feb 06-Feb

29-Jan 28-Jan 02-Feb 30-Jan 28-Jan 28-Jan 28-Jan 28-Jan 28-Jan 29-Jan 28-Jan 27-Jan 30-Jan 30-Jan 03-Feb 28-Jan 28-Jan 28-Jan 21-Jan 29-Jan 03-Feb 29-Jan 02-Feb 30-Jan 28-Jan 29-Jan 29-Jan 28-Jan 29-Jan 02-Feb 28-Jan 28-Jan 03-Feb 28-Jan 28-Jan 29-Jan 28-Jan 28-Jan 03-Feb 31-Jan 02-Feb 28-Jan 28-Jan 30-Jan 29-Jan 03-Feb 28-Jan 02-Feb 29-Jan 02-Feb 29-Jan 29-Jan 28-Jan 28-Jan 01-Feb 28-Jan 28-Jan 28-Jan 02-Feb 28-Jan 28-Jan 28-Jan 29-Jan 02-Apr 29-Jan 02-Feb 28-Jan 28-Jan 28-Jan 02-Feb 28-Jan 29-Jan 28-Jan 28-Jan

03-Feb 02-Feb 09-Feb 30-Jan 29-Jan 28-Jan 02-Feb 28-Jan 02-Feb 05-Feb 28-Jan 27-Jan 09-Feb 03-Feb 05-Feb 28-Jan 28-Jan 16-Feb 02-Feb 29-Jan 04-Feb 30-Jan 04-Feb 03-Feb 29-Jan 29-Jan 05-Feb 30-Jan 04-Feb 05-Feb 28-Jan 28-Jan 06-Feb 29-Jan 28-Jan 02-Feb 30-Jan 30-Jan 06-Feb 09-Feb 02-Feb 29-Jan 29-Jan 10-Feb 04-Feb 10-Feb 02-Feb 02-Feb 12-Feb 10-Feb 03-Feb 02-Feb 30-Jan 28-Jan 04-Feb 02-Feb 02-Feb 03-Feb 12-Feb 30-Jan 29-Jan 29-Jan 30-Jan 02-May 30-Jan 13-Feb 29-Jan 03-Feb 28-Jan 04-Feb 30-Jan 29-Jan 29-Jan 28-Jan

High Resolution Typing HLA-B*27 alleles 1B01/2015 1B02/2015

Comments

PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSP PCR-SSO PCR-SSP Technical problems PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO RT-PCR Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP/Flow Cytometry RT-PCR PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry Euro Array PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP RT-PCR Cytotoxicity PCR-SSO/Flow Cytometry PCR-SSO Cytotoxicity/PRC-SSP Flow Cytometry RT-PCR Flow Cytometry PCR-SSP RT-PCR PCR-SSP Cytotoxicity PCR-SSP Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSP RT-PCR RT-PCR Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSP/MicroArray Cytotoxicity Cytotoxicity Flow Cytometry Flow Cytometry RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP RT-PCR Flow Cytometry PCR-SSP Flow Cytometry

Page 1 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

UK NEQAS for H&I SCHEME 1B -B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B01&02/2015 DESPATCHED ON 27TH JANUARY 2015 Lab No 3 7 8 10 17 20 21 27 31 32 40 57 67 68 83 84 90 93 103 104 106 110 111 128 137 153 198 256 288 295 310 324 326

Monoclonal 1 Designation

Monoclonal 1 Specificity

Monoclonal 1 Manufacturer

Monoclonal 2 Designation

Monoclonal 2 Specificity

Monoclonal 2 Manufacturer

HLA-B27 FITC HLA-B27 Duraclone B27 HLA-B27 145.2 B27-FITC B27-FITC/CD3-PE Duraclone B27 PE MSIgG1 RDI/MsIgG2a FITC PF/FITC HLA-B27 FITC IgG2a B27 HLA-B27 FITC CD3 B7/B27 HLA-B27 Duraclone B27 Iotest HLA-B27 FITC Anti-HLA-B27 HLA-B27 Anti-HLA-B27 FITC Anti-HLA-B27 FITC Iotest HLA-B27 FITC HLA-ABC m3/B27 HLA-B27 FITC CD3/HLA B27 Anti-HLA-B27 Anti-HLA-B27 Anti-HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE

Clone GS145.2 FITC CD3, HLA-B27, HLA-B7 HLA-B27 B27 B27/Weak B7 CD3, HLA-B27, HLA-B7 B27 Isotype control B7/B27

BD BD Beckman Coulter BD One Lambda BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Immunotech Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD One Lambda One Lambda BD Beckman Coulter Immunotech One Lambda BD BD BD BD Beckman Coulter Beckman Coulter Beckman Coulter

CD3 PE CD3

Clone SK7 PE

BD BD

CD3 SK3 CD3-PE B27-FITC

CD3 CD3 B27

BD BD One Lambda

HLA-B27 FITC/B7 PE PE/FITC HLA-B7 PE IgG2b B7 HLA-B7 PE HLA_B27

B7/B27 IgG/IgG2

CD3 B27 F50X Iotest HLA-B7 PE

HLA-B27 FITC PCS B7PE/B27FITC R-PE HLA-B27 HLA-B27 FITC/CD3 PE HLA-B27 FD705-9E1E10 Clone GS145.2 HLA-B27 HLA-B27/B7 IgG2b FD705 HLA-B27 HLA-B27 FITC Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7

Monoclonal 3 Designation

Monoclonal 3 Specificity

Monoclonal 3 Manufacturer

FD705 9E1

HLA-B27

One Lambda

Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter

IgG1K

CF3

Beckman Coulter

FITC

Immunotech Beckman Coulter

HLA-B27 FITC HLA-B7

PE

One Lambda Beckman Coulter

PE HLA-B27 HLA-B7

Beckman Coulter One Lambda Beckman Coulter

B7

Clone BB7.1

HLA-B27 FITC/B7 PE

HLA-B7/B27

Immunotech

CD3 PE Iotest HLA-B7 PE MPC-11 HLA-B27/B7 PE HLA-B7/B27 Anti-CD3 Anti-CD3 Anti-CD3 PE Iotest HLA-B27 FITC/B7 PE H:A-B7 PE MoAb - B27 FITC

Clone SK7 HLA-B7 HLA-B27 IgG2a-IgG1 Crossreaction CD3 PE Clone SK7 HLA-B7 HLA-B7 HLA-B27

BD Beckman Coulter One Lambda Immunotech Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter One Lambda

CD45 PerCp-Cy5.5

Lymphocytes gating

Anti-HLA-B7

Beckman Coulter

HLA-B27 PE

Page 2 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

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