Scheme 1B - 2015-03-04


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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B03&04/2015 DESPATCHED ON 14TH APRIL 2015

Summary of Results Total tested Positive Negative NT/Equivocal % Positive % Negative

111 0 111 1 0.0% 100.0%

110 107 3 2 97.3% 2.7%

Consensus

Negative

Positive

Lab No

Result 1B03/2015

Result 1B04/2015

Date received

Date tested

1 3 4 7 8 9 10 11 12 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 31 32 34 35 37 39 40 41 42 45 46 48 54 55 57 58 62 66 67 68 78 83 84 85 86 90 93 96 101 103 104 105 106 107 108 110 111 112 113 115 126 127 128 129 130 137 142 153 154 156 174 176 185 198 199 209 219 223 225 232 234 236 253 255 256 257 269 270 273 276 278 279 285 288 295 298 300 305 306 307 310 317 319 324 325 326 327 329

Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Equivocal Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative

Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive

17-Apr 15-Apr 15-Apr 15-Apr 15-Apr 16-Apr 16-Apr 13-Apr

06-May 15-Apr 30-Apr 15-Apr 16-Apr 17-Apr 16-Apr 22-Apr 30-Apr 20-Apr 20-Apr 22-Apr 21-Apr 23-Apr 15-Apr 16-Apr 15-Apr 16-Apr 20-Apr 20-Apr 15-Apr 16-Apr 21-Apr 15-Apr 15-Apr 15-Apr 16-Apr 20-Apr 23-Apr 16-Apr 15-Apr 17-Apr 17-Apr 21-Apr 16-Apr 17-Apr 17-Apr 23-Apr 16-Apr 24-Apr 22-Apr 21-Apr 16-Apr 30-Apr 22-Apr 15-Apr 23-Apr 16-Apr 20-Apr 16-Apr 29-Apr 30-Apr 29-Apr 21-Apr 16-Apr 28-Apr 17-Apr 21-Apr 21-Apr 16-Apr 16-Apr 28-Apr 16-Apr 17-Apr 22-Apr 17-Apr 23-Apr 27-Apr 15-Apr 15-Apr 16-Apr 15-Apr 20-Apr 16-Apr 05-May 20-Apr 17-Apr 17-Apr 22-Apr 20-Apr 22-Apr 20-Apr 23-Apr 28-Apr 21-Apr 21-Apr 24-Apr 20-Apr 20-Apr 27-Apr 20-Apr

Donor HLA type Donor HLA type

15-Apr 15-Apr 15-Apr 16-Apr 15-Apr 15-Apr 14-Apr 15-Apr 15-Apr 16-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 16-Apr 15-Apr 15-Apr 16-Apr 15-Apr 14-Apr 16-Apr 15-Apr 17-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 16-Apr 16-Apr 15-Apr 15-Apr 16-Apr 15-Apr 17-Apr 15-Apr 15-Apr 16-Apr 16-Apr 27-Apr 16-Apr 20-Apr 16-Apr 15-Apr 15-Apr 17-Apr 16-Apr 15-Apr 20-Apr 15-Apr 15-Apr 22-Apr 15-Apr 15-Apr 15-Apr 15-Apr 15-Apr 16-Apr 25-Apr 20-Apr 14-Apr 16-Apr 17-Apr 15-Apr 17-Apr 15-Apr 20-Apr 17-Apr 20-Apr 21-Apr 15-Apr 15-Apr 15-Apr 15-Apr 17-Apr 16-Apr 15-Apr 20-Apr 15-Apr 15-Apr 15-Apr 15-Apr 20-Apr 17-Apr 15-Apr 16-Apr 15-Apr 15-Apr 16-Apr 16-Apr 15-Apr 15-Apr 20-Apr 15-Apr

Method

Melting curve Flow Cytometry PCR-SSP, Melting curve Flow Cytometry Flow Cytometry PCR-SSP/SSO Flow Cytometry PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP/Melting curve PCR-SSP PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSO PCR-SSP/SSO Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO RT-PCR Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP/Flow Cytometry RT-PCR PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry Euro Array PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP RT-PCR Cytotoxicity PCR-SSO/Flow Cytometry PCR-SSO Cytotoxicity/PRC-SSP Flow Cytometry RT-PCR Flow Cytometry PCR-SSP RT-PCR PCR-SSO Cytotoxicity PCR-SSP Flow Cytometry RT-PCR PCR-SSP RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry RT-PCR RT-PCR Flow Cytometry PCR-SSO PCR-SSP PCR-SSP 24-Apr PCR-SSP 20-Apr PCR-SSP 23-Apr MicroArray/SSP 16-Apr Cytotoxicity 16-Apr Cytotoxicity 16-Apr Flow Cytometry 17-Apr Flow Cytometry 18-May RT-PCR 18-Apr PCR-SSP 17-Apr PCR-SSP 16-Apr PCR-SSP 21-Apr PCR-SSP 15-Apr Flow Cytometry 15-Apr PCR-SSP 17-Apr 16-Apr Flow Cytometry 16-Apr PCR-SSP 16-Apr Flow Cytometry 22-Apr PCR-SSP 29-Apr PCR-SSO

1B03/2015 1B04/2015

HLA-A2, A3; B7, B13; Cw6, Cw7; DR15, DR7; DQ2, DQ6 HLA-A1, A2; B8, B27; Cw1, Cw7; DR7, DR17; DQ2, -

High Resolution Typing HLA-B*27 alleles 1B03/2015 1B04/2015

Comments

B*27:05/47/116/117

Sample 1B04/2015 heterozygous positive for HLA B27

Sample 1B03/2015 is HLA-B7 positive

HLA-B7 cross-reaction

Page 1 of 1 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING

HLA-B27 TYPING RESULTS OF SAMPLES 1B03&02/2015 DESPATCHED ON 14TH APRIL 2015 Lab No 3 7 8 10 20 21 27 31 32 40 46 57 67 68 83 84 90 93 103 104 106 110 111 128 137 153 198 236 256 288 295 310 324 326

Monoclonal 1 Designation

Monoclonal 1 Specificity

Monoclonal 1 Manufacturer

Monoclonal 2 Designation

Monoclonal 2 Specificity

Monoclonal 2 Manufacturer

HLA-B27 FITC HLA-B27 Duraclone B27 HLA-B27 145.2 B27-FITC/CD3-PE Duraclone B27 Duraclone B27 MSIgG1 RDI/MsIgG2a FITC PF/FITC HLA-B27 FITC CD3 IgG2a B27 HLA-B27 FITC CD3 B7/B27 HLA-B27 Duraclone B27 Iotest HLA-B27 FITC Anti-HLA-B27 HLA-B27 Anti-HLA-B27 FITC Anti-HLA-B27 FITC Iotest HLA-B27 FITC HLA-ABC m3/BB7.1 HLA-B27 FITC CD3/HLA B27 HLA-B27 FITC Anti-HLA-B27 Anti-HLA-B27 Anti-HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE

Clone GS145.2 FITC CD3, HLA-B27, HLA-B7 HLA-B27 B27/Weak B7 CD3, HLA-B27, HLA-B7 CD3, HLA-B27, HLA-B7 Isotype control B7/B27

BD BD Beckman Coulter BD BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Immunotech Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD One Lambda One Lambda BD Beckman Coulter Immunotech One Lambda BD Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter Beckman Coulter

CD3 PE CD3

Clone SK7 PE

BD BD

CD3 SK3 B27-FITC

CD3 B27

BD One Lambda

HLA-B27 FITC/B7 PE PE/FITC HLA-B7 PE Anti-HLA-B27 IgG2b B27 HLA-B7 PE HLA-B27

B7/B27 IgG/IgG2

PE HLA-B27 ABCm3 PCS B7PE/B27FITC R-PE HLA-B27 HLA-B27 FITC/CD3 PE HLA-B27 FD705-9E1E10 Clone GS145.2 HLA-B27 HLA-B27/B7 IgG2b FD705 HLA-B27 HLA-ABC-m3 HLA-B27 FITC Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7

FITC

Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Immunotech Beckman Coulter

CD3 B27 F50X Iotest HLA-B7 PE

PE HLA-B27 HLA-B7

Beckman Coulter One Lambda Beckman Coulter

HLA-B27

HLA-B7/B27

Immunotech

CD3 PE Iotest HLA-B7 PE MPC-11

Clone SK7 HLA-B7 HLA-B27

BD Beckman Coulter One Lambda

HLA-B7/B27 HLA-B7 PE Anti-CD3 Anti-CD3 Anti-CD3 PE Iotest HLA-B27 FITC/B7 PE HLA-B7 PE MoAb - B27 FITC

Crossreaction BB7.1 CD3 PE Clone SK7 HLA-B7 HLA-B7 HLA-B27

Beckman Coulter Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter One Lambda

FITC HLA-B27 FD705

Monoclonal 3 Designation

Monoclonal 3 Specificity

Monoclonal 3 Manufacturer

FD705 9E1

HLA-B27

One Lambda

IgG1K B7 HLA-B27 FITC HLA-B7

CF3 BB7.1

Beckman Coulter Beckman Coulter One Lambda Beckman Coulter

B7

Clone BB7.1

CD45 PerCp-Cy5.5

Lymphocytes gating

BD

Anti-HLA-B7

PE

Beckman Coulter

Page 1 of 1 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

PE