Scheme 1B - 2015-05-06

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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B05&06/2015 DESPATCHED ON 09TH JUNE 2015

Summary of Results Total tested Positive Negative NT/Equivocal % Positive % Negative

111 1 110 2 0.9% 99.1%

109 107 2 4 98.2% 1.8%

Consensus

Negative

Positive

Lab No

Result 1B05/2015

Result 1B06/2015

Date received

Date tested

Method

1 3 4 7 8 9 10 11 12 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 31 32 34 35 37 39 40 41 42 45 46 48 54 55 57 58 62 66 67 68 78 83 84 85 86 90 93 96 101 103 104 106 107 108 110 111 112 113 115 126 127 128 129 130 131 137 142 153 154 156 174 176 185 198 199 209 219 223 225 232 234 236 245 253 255 256 257 269 273 276 278 279 285 288 295 298 300 305 306 307 310 317 319 324 325 326 327 329 334

Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative

Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive NT Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive NT Positive Positive Negative Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive

12-Jun 10-Jun 10-Jun 11-Jun 10-Jun 10-Jun 10-Jun 08-Jun 10-Jun 10-Jun 10-Jun 11-Jun 18-Jun 12-Jun 10-Jun 11-Jun 10-Jun 11-Jun 11-Jun 10-Jun 10-Jun 11-Jun 10-Jun 11-Jun

15-Jun 10-Jun 18-Jun 11-Jun 10-Jun 12-Jun 10-Jun 17-Jun 19-Jun 12-Jun 15-Jun 25-Jun 26-Jun 15-Jun 11-Jun 11-Jun 11-Jun 26-Jun 15-Jun 12-Jun 11-Jun 11-Jun 15-Jun 16-Jun

Melting Curve Analysis Flow Cytometry PCR-SSP, Melting curve Flow Cytometry Flow Cytometry PCR-SSP/SSO Flow Cytometry PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSP, Flow Cytometry PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP/Melting curve PCR-SSP PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSO PCR-SSP/SSO

10-Jun 11-Jun 10-Jun 11-Jun 10-Jun 11-Jun 11-Jun 10-Jun 10-Jun 11-Jun 10-Jun 10-Jun 10-Jun 11-Jun 11-Jun 10-Jun 10-Jun 10-Jun 12-Jun 10-Jun 10-Jun 12-Jun 11-Jun 15-Jun 10-Jun 10-Jun 10-Jun 10-Jun 10-Jun 12-Jun 16-Jun 12-Jun 10-Jun 11-Jun 19-Jun 10-Jun 10-Jun 15-Jun 10-Jun 10-Jun 17-Jun 11-Jun 10-Jun 10-Jun 10-Jun 10-Jun 10-Jun 09-Jun 25-Jun 15-Jun 10-Jun 10-Jun 15-Jun 06-Jun 15-Jun 10-Jun

12-Jun 12-Jun 15-Jun 18-Jun 17-Jun 10-Jun 17-Jun 16-Jun 11-Jun 10-Jun 16-Jun 16-Jun 12-Jun 10-Jun 19-Jun 24-Jun 16-Jun 10-Jun 11-Jun 16-Jun 11-Jun 10-Jun 17-Jun 12-Jun 16-Jun 11-Jun 12-Jun 16-Jun 15-Jun 11-Jun 15-Jun 19-Jun 15-Jun 11-Jun 11-Jun 26-Jun 15-Jun 15-Jun 17-Jun 12-Jun 10-Jun 22-Jun 11-Jun 15-Jun 10-Jun 11-Jun 11-Jun 18-Jun 16-Jun 27-Jun 15-Jun 11-Jun 11-Jun 19-Jun 12-Jun 16-Jun 16-Jun

Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO RT-PCR Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP/Flow Cytometry RT-PCR PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry Flow Cytometry Euro Array PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP RT-PCR Cytotoxicity PCR-SSO/Flow Cytometry PCR-SSO Cytotoxicity/PRC-SSP Flow Cytometry Flow Cytometry RT-PCR Flow Cytometry PCR-SSP RT-PCR PCR-SSO Cytotoxicity PCR-SSP Flow Cytometry RT-PCR PCR-SSP RT-PCR PCR-SSP

15-Jun 15-Jun 16-Jun 12-Jun 10-Jun 10-Jun 10-Jun 11-Jun 24-Jun 11-Jun 10-Jun 15-Jun 10-Jun 10-Jun 10-Jun 12-Jun 15-Jun 13-Jun 10-Jun 10-Jun 11-Jun 10-Jun 15-Jun 10-Jun 12-Jun 11-Jun 12-Jun 15-Jun 10-Jun 10-Jun

24-Jun 16-Jun 17-Jun 16-Jun 11-Jun 15-Jun 11-Jun 11-Jun 26-Jun 15-Jun 17-Jun 16-Jun 10-Jun 11-Jun 10-Jun 13-Jun 18-Jun 20-Jun 26-Jun 11-Jun 19-Jun 10-Jun 15-Jun 10-Jun 15-Jun 11-Jun 12-Jun 17-Jun 26-Jun 11-Jun

PCR-SSP PCR-SSP Flow Cytometry PCR-SSO RT-PCR RT-PCR Flow Cytometry PCR-SSO PCR-SSP PCR-SSP PCR-SSP MicroArray/SSP Cytotoxicity Cytotoxicity Flow Cytometry Flow Cytometry RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP

Donor HLA type Donor HLA type

1B05/2015 1B06/2015

HLA-A2, A31; B7, B51; Cw7, Cw15; DR14, DR15; DQ5, DQ6 HLA-A2, A26; B27, B73; Cw2, Cw15; DR4, DR17; DQ2, DQ8

High Resolution Typing HLA-B*27 alleles 1B05/2015 1B06/2015

Comments

Samples not received

B27*05:47/116/117

Samples not received

Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP PCR-SSO Flow Cytometry

Page 1 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING

HLA-B27 TYPING RESULTS OF SAMPLES 1B05&06/2015 DESPATCHED ON 09TH JUNE 2015 Lab No 3 7 8 10 17 20 21 27 31 32 40 46 57 67 68 83 84 90 93 103 104 106 110 111 128 131 137 153 198 236 256 288 295 310 324 326 334

Monoclonal 1 Designation

Monoclonal 1 Specificity

Monoclonal 1 Manufacturer

Monoclonal 2 Designation

Monoclonal 2 Specificity

Monoclonal 2 Manufacturer

HLA-B27 FITC HLA-B27 Duraclone B27 HLA-B27 145.2 B-27 FITC B27-FITC/CD3-PE Duraclone B27 Duraclone B27 MSIgG1 RDI/MsIgG2a FITC PF/FITC HLA-B27 FITC CD3 IgG2a B27 HLA-B27 FITC CD3 B7/B27 HLA-B27 Duraclone B27 Iotest HLA-B27 FITC Anti-HLA-B27 HLA-B27 Anti-HLA-B27 FITC Anti-HLA-B27 FITC Iotest HLA-B27 FITC Iotest HLA-B27 FITC HLA-ABC m3/BB7.1 HLA-B27 FITC CD3/HLA B27 HLA-B27 FITC Anti-HLA-B27 Anti-HLA-B27 Anti-HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC

Clone GS145.2 FITC CD3, HLA-B27, HLA-B7 HLA-B27 B27/Weak B7 B27/Weak B7 CD3, HLA-B27, HLA-B7 CD3, HLA-B27, HLA-B7 Isotype control B7/B27

BD BD Beckman Coulter BD One Lambda BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Immunotech Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD One Lambda One Lambda BD Beckman Coulter Beckman Coulter Immunotech One Lambda BD Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter

CD3 PE CD3

Clone SK7 PE

BD BD

CD3 SK3 CD3-PE B27-FITC

CD3 CD3 B27

BD BD One Lambda

HLA-B27 FITC/B7 PE PE/FITC HLA-B7 PE Anti-HLA-B27 IgG2b B27 HLA-B7 PE HLA-B27

B7/B27 IgG/IgG2

FITC

Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Immunotech Beckman Coulter

PE HLA-B27 HLA-B7

Beckman Coulter One Lambda Beckman Coulter

PE HLA-B27 ABCm3 PCS B7PE/B27FITC R-PE HLA-B27 HLA-B27 FITC/CD3 PE HLA-B27 FD705-9E1E10 Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7 IgG2b FD705 HLA-B27 HLA-ABC-m3 HLA-B27 FITC Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7

CD3 B27 F50X Iotest HLA-B7 PE

FITC HLA-B27 FD705

HLA-B27

HLA-B7/B27

Immunotech

CD3 PE Iotest HLA-B7 PE Iotest HLA-B7 PE MPC-11

Clone SK7 HLA-B7 HLA-B7 HLA-B27

BD Beckman Coulter Beckman Coulter One Lambda

HLA-B7/B27 HLA-B7 PE Anti-CD3 Anti-CD3 Anti-CD3 PE Iotest HLA-B27 FITC/B7 PE HLA-B7 PE MoAb - B27 FITC HLA-B7 PE

Crossreaction BB7.1 CD3 PE Clone SK7 HLA-B7 HLA-B7 HLA-B27

Beckman Coulter Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter One Lambda Beckman Coulter

Monoclonal 3 Designation

Monoclonal 3 Specificity

Monoclonal 3 Manufacturer

FD705 9E1

HLA-B27

One Lambda

IgG1K B7 HLA-B27 FITC HLA-B7

CF3 BB7.1

Beckman Coulter Beckman Coulter One Lambda Beckman Coulter

B7

Clone BB7.1

CD45 PerCp-Cy5.5

Lymphocytes gating

BD

Anti-HLA-B7

PE

Beckman Coulter

Page 2 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

PE