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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B09+10/2015 DESPATCHED ON 08TH SEPTEMBER 2015
Summary of Results Total tested (excl. Positive Negative NT/Equivocal % Positive % Negative Consensus Lab No 1 3 4 7 8 9 10 11 12 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 31 32 34 35 37 39 40 41 42 45 46 48 54 55 57 58 62 66 67 68 71 72 78 83 84 85 86 90 93 96 101 103 104 106 107 108 110 111 112 113 115 126 127 128 129 130 131 137 142 153 154 156 174 176 185 198 199 209 219 223 225 232 234 236 245 253 255 256 257 269 273 276 278 279 285 288 295 298 300 305 306 307 310 317 319 324 325 326 327 329 334
115 114 1 0 99.1% 0.9%
112 112 0 3 100.0% 0.0%
Positive
Positive
Result 1B09/2015 Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive
Result 1B10/2015 Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive
Donor HLA type Donor HLA type
Date received 09-Sep 10-Sep 09-Sep 14-Sep 10-Sep 09-Sep 09-Sep 07-Sep 09-Sep 09-Sep 09-Sep 09-Sep 21-Sep 11-Sep 10-Sep 10-Sep 09-Sep 09-Sep 09-Sep 10-Sep 09-Sep 11-Sep 09-Sep 09-Sep 09-Sep 10-Sep 09-Sep 10-Sep 09-Sep 09-Sep 10-Sep 09-Sep 09-Sep 10-Sep 10-Sep 10-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 11-Sep 10-Sep 10-Sep 09-Sep 10-Sep 09-Sep 11-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 10-Sep 14-Sep 10-Sep 09-Sep 10-Sep 12-Sep 09-Sep 09-Sep 14-Sep 09-Sep 10-Sep 23-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 09-Sep 12-Sep 15-Sep 09-Sep 09-Sep 11-Sep 09-Sep 09-Sep 14-Sep 11-Sep 14-Sep 15-Sep 09-Sep 11-Sep 09-Sep 09-Sep 10-Sep 16-Sep 09-Sep 09-Sep 14-Sep 09-Sep 09-Sep 09-Sep 09-Sep 14-Sep 10-Sep 15-Sep 09-Sep 09-Sep 09-Sep 14-Sep 04-Sep 10-Sep 09-Sep 09-Sep 14-Sep 09-Sep 09-Sep
Date tested 18-Sep 11-Sep 25-Sep 15-Sep 10-Sep 09-Sep 16-Jun 21-Sep 11-Sep 10-Sep 22-Sep 22-Sep 15-Sep 11-Sep 10-Sep 11-Sep 15-Sep 11-Sep 15-Sep 10-Sep 16-Sep 14-Sep 11-Sep 09-Sep 10-Sep 10-Sep 11-Sep 17-Sep 14-Sep 11-Sep 15-Sep 15-Sep 15-Sep 10-Sep 14-Sep 10-Sep 15-Sep 09-Sep 15-Sep 23-Sep 15-Sep 10-Sep 09-Sep 10-Sep 26-Sep 15-Sep 11-Sep 23-Sep 17-Sep 10-Sep 14-Sep 15-Sep 23-Sep 15-Sep 11-Sep 09-Sep 15-Sep 15-Sep 14-Sep 09-Sep 10-Sep 28-Sep 16-Sep 11-Sep 23-Sep 11-Sep 15-Sep 24-Sep 14-Sep 11-Sep 09-Sep 17-Sep 15-Sep 11-Sep 14-Sep 25-Sep 15-Sep 10-Sep 09-Sep 15-Sep 15-Sep 11-Sep 14-Sep 15-Sep 15-Sep 15-Sep 15-Sep 14-Sep 25-Sep 15-Sep 09-Sep 10-Sep 17-Sep 15-Sep 14-Sep 21-Sep 11-Sep 09-Sep 09-Sep 23-Sep 17-Sep 14-Sep 22-Sep 10-Sep 21-Sep 07-Oct 26-Sep 11-Sep 10-Sep 10-Sep 11-Sep 22-Sep 25-Sep 10-Sep
Method
1B09/2015 1B10/2015
HLA-A2, A-; B27, B62; Cw2, Cw10; DR1, DR12, DQ5, DQ7 HLA-A2, A30; B18, B27; Cw1, Cw5; DR1, DR17, DQ2, DQ5
High Resolution Typing HLA-B*27 alleles 1B09/2015 1B10/2015
Melting Curve Analysis Flow Cytometry PCR-SSP, Melting curve Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSO PCR-SSP PCR-SSO PCR-SSO PCR-SSP PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP/Melting curve PCR-SSP PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSO PCR-SSP/SSO Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSO PCR-SSP Flow Cytometry PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSP/SBT RT-PCR Flow Cytometry Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry PCR-SSP/Flow Cytometry RT-PCR PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry Flow Cytometry MicroArray PCR-SSP PCR-SSP/Flow Cytometry Flow Cytometry PCR-SSP PCR-SSO PCR-SSP RT-PCR MicroArray PCR-SSO/Flow Cytometry PCR-SSO Cytotoxicity/PRC-SSP Flow Cytometry Flow Cytometry RT-PCR Flow Cytometry PCR-SSP RT-PCR PCR-SSO Cytotoxicity PCR-SSP Flow Cytometry RT-PCR PCR-SSP RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSO RT-PCR RT-PCR Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP MicroArray/SSP Cytotoxicity Cytotoxicity Flow Cytometry Flow Cytometry RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSO Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP PCR-SSO Flow Cytometry
Comments
1B10/2015 would be sent away for PCR confirmation
B*27:05/47/116/117
B*27:05/47/116/117
Page 1 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
1B v1 12/08/15
UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B09+10/2015 DESPATCHED ON 08TH SEPTEMBER 2015 Lab No 3 7 8 10 20 21 27 31 32 40 46 57 67 68 71 83 84 90 93 103 104 106 110 111 128 131 137 153 198 236 256 288 295 310 324 326 334
Monoclonal 1 Designation HLA-B27 FITC HLA-B27 Duraclone B27 HLA-B27 145.2 B27-FITC HLA-B27 Duraclone B27 MSIgG1 RDI/MsIgG2a FITC PF/FITC HLA-B27 PE CD3 IgG2a ABC m3 HLA-B27/B7 Duraclone B27 HLA-B27 B7/B27 HLA-B27 FITC Iotest HLA-B27 FITC Anti-HLA-B27 HLA-B27 Anti-HLA-B27 FITC Anti-HLA-B27 FITC Iotest HLA-B27 FITC Iotest HLA-B27 FITC HLA-ABC m3/BB7.1 HLA-B27 FITC HLA-B27 testing HLA-B27 FITC Anti-HLA-B27 Anti-HLA-B27 Anti-HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC Iotest HLA-B27 FITC/B7 PE HLA-B27 FITC
Monoclonal 1 Specificity Clone GS145.2 FITC CD3, HLA-B27, HLA-B7 HLA-B27 B27 HLA-B27 FITC CD3, HLA-B27, HLA-B7 Isotype control B7/B27 PE HLA-B27 HLA-B27 HLA-B27-FITC/B7-PE B27 FITC B7/B27 R-PE B27 HLA-B27 FITC/CD3/PE HLA-B27 FD705-9E1E10 Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7 IgG2b FD705 HLA-B27 +CD3 HLA-ABC-m3 HLA-B27 FITC Clone GS145.2 HLA-B27 HLA-B27 HLA-B27
Monoclonal 1 Manufacturer BD BD Beckman Coulter BD BD One Lambda Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter One Lambda Beckman Coulter BD One Lambda One Lambda BD Beckman Coulter Beckman Coulter Immunotech One Lambda BD Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter
Monoclonal 2 Designation CD3 PE CD3
Monoclonal 2 Specificity Clone SK7 PE
Monoclonal 2 Manufacturer BD BD
Monoclonal 3 Designation
Monoclonal 3 Specificity
Monoclonal 3 Manufacturer
CD3 SK3 B27-FITC Iotest
CD3 B27 HLA-B27-FITC/HLA-B7-PE
BD One Lambda Beckman Coulter
FD705 9E1
HLA-B27
One Lambda
HLA-B27 FITC/B7 PE PE/FITC CD3-PE-DY649 Anti-HLA-B27 IgG2b FD705 HLA-B27
B7/B27 IgG/IgG2
Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter One Lambda
IgG1K BB7.1
CF3 HLA-B27
Beckman Coulter Beckman Coulter
FITC HLA-B27 HLA-B27 HLA-B27-FITC
HLA-B7
PE
Beckman Coulter
CD3
PC5
Beckman Coulter
CD3 Duraclone Iotest HLA-B7 PE
PE B27 HLA-B7
Beckman Coulter Beckman Coulter Beckman Coulter
B7
Clone BB7.1
Beckman Coulter
HLA-B27
HLA-B7/B27
Immunotech
CD3 PE Iotest HLA-B7 PE Iotest HLA-B7 PE MPC-11 HLA-B27-FITC/B7-PE HLA-B27 control
Clone SK7 HLA-B7 HLA-B7 HLA-B27 IgG2a-IgG1 HLA-B27
BD Beckman Coulter Beckman Coulter One Lambda Immunotech Beckman Coulter
CD45 PerCp-Cy5.5
Lymphocytes gating
BD
HLA-B27 cross
HLA-B7
Beckman Coulter
Anti-CD3 Anti-CD3 Anti-CD3 PE Iotest HLA-B27 FITC/B7 PE HLA-B7 PE MoAb - B27 FITC HLA-B7 PE
CD3 PE Clone SK7 HLA-B7 HLA-B7 HLA-B27
BD BD BD Beckman Coulter Beckman Coulter One Lambda Beckman Coulter
Anti-HLA-B7
PE
Beckman Coulter
Page 2 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, no data may be published without permission from UK NEQAS for H&I
1B v1 12/08/15
