[PDF]UK NEQAS for H&I Educational Scheme UK NEQAS...
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UK NEQAS for H&I Educational Scheme METHODOLOGY DESPATCHED ON 10TH OCTOBER 2017 SAMPLE ED04/2017 Lab No. 9 12 15 20 23 24 26 29 38 39 41 42 48 54 58 62 112 113 130 132 133 142 147 156 164 181 194 220 238 260 262 267 284 292 315
Date
16-Oct 23-Oct 13-Oct 13-Oct 18-Oct 17-Oct 13-Oct 13-Oct 13-Oct 12-Oct 20-Oct 18-Oct 31-Oct 02-Nov 18-Oct 11-Oct 19-Oct 18-Oct 19-Oct 31-Oct 17-Oct 18-Oct 16-Oct 23-Oct 12-Oct 25-Oct 13-Oct 31-Oct 23-Oct 21-Oct 16-Oct 25-Oct 26-Oct 18-Oct 18-Oct
Method used * A SSOP/SBT/LUM NGS LUM SSP LUM LUM LUM
B SSOP/SBT/LUM NGS LUM/SBT SSP/SBT LUM/SBT LUM/SBT LUM/SBT
C DRB1 DRB3,4,5 SSOP/SBT/LUM SSOP/SBT/LUM SSP/SSOP/LUM NGS NGS NGS LUM LUM LUM SSP LUM LUM LUM LUM LUM LUM LUM LUM
LUM
DQA1 DQB1 DPB1 SSOP/LUM SSOP/SBT/LUM NGS NGS NGS LUM LUM LUM SSP LUM LUM LUM LUM
LUM NGS LUM SSP/SBT
LUM/SBT NGS LUM SSP/SBT
LUM NGS LUM SSP/SBT
LUM NGS LUM SSP/SBT
LUM NGS LUM
LUM SBT/NGS
LUM SSP/SBT/NGS
LUM SBT/NGS
LUM SBT/NGS
LUM/NGS SBT SSP/NGS
LUM/NGS SBT SSP/NGS
LUM/NGS SBT SSP/NGS
NGS SSP
SSP
LUM/NGS SBT SSP/NGS SBT SSP/SBT
SSP
SSP
SSP
LUM SSOP/SBT NGS LUM
LUM/SSP SSOP/SBT/LUM NGS LUM
LUM SSOP/SBT/LUM NGS LUM
LUM SSOP/SBT NGS LUM
LUM In-house NGS
LUM
LUM LUM LUM SSP
LUM/SBT LUM/SBT LUM SSP
LUM LUM LUM SSP
LUM LUM LUM SSP
SSP LUM SBT
LUM SSP
LUM NGS LUM SSP/SBT LUM SBT/NGS
SBT/NGS
SSP/NGS
LUM/NGS SBT SSP/NGS
SSP/NGS
SSP
SSP
SSP
NGS LUM
LUM LUM
SSOP NGS LUM LUM LUM LUM SSP
Source of Primers and Probes Commercial In-house Other Kits YES YES YES YES YES YES YES
Detection Method
Dynal, One Lambda, Secore Illumina Immucor
Gel, Fluorescence Fluorescence Fluorescence Gel
One Lambda Immucor, GenDx
Fluorescence
YES
One Lambda, Olerup, BAG Health Care
Gel, Fluorescence, Reverse dot-blot assay
YES YES YES YES
YES
One Lambda, Gendx Omixon, Illumina One Lambda Life Technologies - ALLSET GOLD, SeeCORE
Fluorescence Illumina Miseq Fluorescence Gel
YES YES
YES
One Lambda Olerup, Lifetech. PACBio
Fluorescence Gel, Fluorescence
One Lambda. GENDX/ILLUMINA GenDx Illumina, HLA Twin, Olerup, Omixon Immucor, GenDx Linkage, Protrans
Gel. ILLUMINA MiSEQ
YES YES YES YES
YES NGS YES YES YES SSP
Kit manufacturer
YES
One Lambda, Olerup BAG, Secore Illumina. Omixon Immucor Immucor, Protrans One Lambda (INGEN), Protrans One Lambda Innotrain
Fluorescence Fluorescence
Mr. Spot, Sanger Sequencing Fluorescence Fluorescence Fluorescence Fluorescence
NEQ-129 Issue 1 UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
P 1 of 3 Effective Date 15/03/16
UK NEQAS for H&I Educational Scheme CLASS I DNA TYPING RESULTS DESPATCHED ON 10TH OCTOBER 2017 SAMPLE ED04/2017 Lab No. 9 12 15 20 23 24 26 29 38 39 41 42 48 54 58 62 112 113 130 132 133 142 147 156 164 181 194 220 238 260 262 267 284 292 315
A*
A*
01:01/01L/30/66/98/100/191 01:01L 01:01/32/37 01:01:38L 01 01:01 01:01:38L 01:01:38L 01:01:38L 01:01 01:01:38L 01:01/01:01:38L 01:01:38L 01:01:38L 01 01:01:38L 01 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01:38L 01:01P 01:01:38L 01 01:01:38L 01 01:01:38L 01 01:01
02:01/01L/01W/246/338/262/346/609/665 02:01 02:01/09/66 02:01/629/642/665 02 02:01 02:01 02:01/629/642/665 02:01:01 02:01 02:01:01:01/02L/16 02:01/02:01:01:02L/02:665 02:01 02:01 02 02:01:01 02 02:01:01 02:01:01:01 02:01:01:01/02:01:01:16/02:01:01:02L 02:01:01 02 02:01:01/02:01:26/02:01:130/02:642 02:01/02:629 02:01:01 02:01:01 02 02:01P 02:01 02 02:01:01 02 02:01:01 02 02:01
B*
B*
C*
C*
15:17 15:17/168/177 15(63) 15 15:17… 15 15 15:17:01 15:17/168/177/196/XX 15:17:01:01 15 15 15 15 15:17:01:01 15 15:17:01 15:17:01 15:17:01:01 15:17:01 15
57:01 57 57 57 57 57 57 57:01:01 57 57:01:01 57 57 57 57 57:01:01 57 57:01:01 57:01:01 57:01:01 57:01:01 57
06:02 06 06 06 06 06 06 06:02:01 06 06:02:01:01 06 06 06 06 06:02:01:01
07:01 07 07 07 07 07 07 07:01:02 07 07:01:02 07 07 07 07 07:01:02
06:02:01 06:02:01:01 06:02:01:01 06:02:01 06
07:01:02 07:01:02 07:01:02 07:01:02 07
15 15:17:01 15:17:01 15 15 15:17 15 15:17:01:01 15 15:17:01:01 15 15:17
57 57:01:01 57:01:01 57 57 57:01 57 57:01:01 57 57:01:01 57 57:01
06 06:02:01 06:02:01 06 06 06:02 06 06:02:01:01 06 06:02:01:01 06 06:02
07 07:01:02 07:01:02 07 07 07:01 07 07:01:02 07 07:01:01G 07 07:01
NEQ-129 Issue 1 UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
P 2 of 3 Effective Date 15/03/16
UK NEQAS for H&I Educational Scheme CLASS II DNA TYPING RESULTS DESPATCHED ON 10TH OCTOBER 2017 SAMPLE ED04/2017 Lab No. 12 15 20 23 24 26 29 38 39 41 42 48 54 58 62 112 113 130 132 133 142 156 164 181 194 220 238 260 262 267 284 292 315
DRB1*
DRB1*
DRB4*
DQA1*
DQA1*
DQB1*
DQB1*
DPA1*
DPA1*
DPB1*
DPB1*
01:01 01:01/05/07 01 01 01:01… 01 01 01:01:01 01 01:01:01 01 01 01 01 01:01:01 01 01:01:01 01:01:01 01:01:01 01:01 01 01 01:01:01 01:01:01 01 01 01:01 01 01:01:01 01 01:01:01 01 01:01
07:01 07 07 07 07 07 07 07:01:01 07 07:01:01:01/02 07 07 07 07 07:01:01 07 07:01:01 07:01:01 07:01:01:01/07:01:01:02 07:01 07 07 07:01:01 07:01:01 07 07 07:01 07 07:01:01 07 07:01:01 07 07:01
01:03N 01:03:01:02N
01:01 01 01 01 01
02:01 02 02:01 02 02
-
04:01
-
02:01:01 02 02:01:01:01
01 01
02 02
01
02
05:01 05 05 05 05 05 05 05:01:01 05 05:01:01:03 05 05 05 05 05:01:01 05 05:01:01 05:01:01:03
01:03
01:01:01 01 01:01:01
03:03 03:03/15/26 03(9) 03 03:03… 03 03 03:03:02 03:03/15/26/30/31/XX 03:03:02:01 03 03 03 03 03:03:02 03 03:03:02 03:03:02:01 03:03:02 03 03 03:03:02 03:03:02 03 03 03:03 03 03:03:02 03
05:01:01 05 05 05:01:01 05:01:01 05 05 05:01 05 05:01:01 05
03 03:03
05 05:01
01:03:01:02N 01/01N…
01:03:01N 01
01:03:01:02N 01 01:03:01:02N 01:01/03/03N/06/09/11/17/21/22/23/26/27/36/37/41/49/02:01N/03:01N + 01:03:01:02N
01:01:01:01/01:01:01:03
02:01:01:02
01:03:01:02N
01
02
01:03:01:02N
01:01:01 01
02:01:01 02:01
01:01 01 01:01:01
02:01 02 02:01:01
01 01
02 02:01
01N
01
01:03:01
Comments
04:01:01 Cannot exclude A*01:01:38L, A*02:01:01:02L and A*02:01:14Q
01
04
04:01:01:01 01
01
04:01:01:06 DQB1 typing failed and as it was not one of the loci of ierest was not repeated
04:01
04:01:01
04:01:01
04:01 04:01:01
04:01:01
NEQ-129 Issue 1 UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
P 3 of 3 Effective Date 15/03/16
