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UK NEQAS for H&I Educational Scheme METHODOLOGY DESPATCHED ON 28/06/2016 SAMPLE ED 04/2016 Lab No. 9 12 14 15 16 19 20 23 24 26 28 29 34 38 39 41 42 45 48 54 58 62 78 112 113 128 130 132 133 142 147 156 159 164 178 181 193 195 229 230 238 245 252 259 260 262 267 284 292 315 323
Date received
Date tested
Method used * HLA-A
29-Jun 28-Jun 29-Jun 30-Jun 29-Jun 01-Jul 29-Jun 28-Jun 30-Jun 30-Jun 29-Jun 30-Jun 30-Jun 29-Jun 29-Jun 29-Jun 29-Jun 29-Jun 29-Jun 29-Jun 29-Jun 30-Jun
30-Jun 06-Jul 07-Jul 06-Jul 05-Jul 13-Jul 04-Jul 15-Jul 07-Apr 30-Jun 05-Jul 01-Jul 01-Jul 30-Jun 01-Jul
SBT/LUM SBT SSP/SBT/LUM SBT SSP/SBT
HLA-B
HLA-C
DRB1
DRB3,4,5
DQA1
DQB1
DPA1
SBT/LUM SSOP SSOP SSP/SBT/LUM
LUM SSOP SSOP LUM
LUM SSOP SSOP LUM
LUM SSOP SSOP LUM
LUM SSOP SSOP SSP/LUM
LUM SSOP SSOP LUM
LUM SSOP SSOP LUM
LUM SSP/SBT LUM LUM SBT/LUM LUM SBT SBT/LUM SBT/LUM SSOP SSOP SBT/LUM SSP/SBT SSP/SBT LUM LUM SSP/SBT/TGS
LUM
LUM
LUM
LUM
LUM
LUM
LUM LUM LUM
LUM LUM LUM
SBT SBT/LUM LUM SSOP
SBT SBT/LUM LUM SSOP
SBT/LUM LUM SSOP
LUM
LUM
LUM
SSP/SBT LUM LUM SSP/SBT/TGS
SSP/SBT LUM LUM SBT/TGS
SSP/SBT LUM LUM
SSP SSP/LUM/NGS LUM SBT NGS LUM RT-PCRSSP SBT/LUM SBT
SSP/LUM/NGS LUM SBT NGS LUM RT-PCRSSP SBT/LUM SBT
SSP SSP/LUM/NGS LUM SBT NGS LUM SBT/RT-PCRSSP SBT/LUM SBT
SSP SSP/LUM/NGS LUM SBT NGS LUM RT-PCRSSP SBT/LUM SBT
SBT LUM SSP/SBT/LUM NGS 13-Jul SSP/LUM 05-Aug LUM 15-Jul SBT 05-Jul SBT
SBT LUM LUM NGS LUM LUM
SSP/SBT LUM LUM NGS LUM LUM
SBT SSP LUM NGS LUM LUM
LUM LUM
LUM LUM
SSP/SBT SBT
SBT SBT
SBT SBT
SBT SBT
SBT SBT
30-Jun
13-Jul
SSOP/LUM
05-Jul 30-Jun 30-Jun 30-Jun 30-Jun 07-Jul
21-Jul 07-May 05-Jul 04-Jul 01-Jul
SSP/SBT/LUM SBT/LUM SBT/LUM SSOP/LUM SSP LUM
SSOP/LUM LUM SSP/SBT/LUM SBT/LUM SBT/LUM SSOP/LUM SSP LUM
SSOP/LUM LUM SSP/SBT/LUM LUM LUM SSOP/LUM SSP LUM
SSOP/LUM LUM SSP/SBT/LUM LUM LUM SSOP/LUM SSP LUM
SSOP/LUM LUM SSP/SBT/LUM LUM LUM SSOP/LUM SSP LUM
07-Jan 29-Jun 29-Jun 29-Jun 29-Jun 01-Jul 01-Jul 28-Jun 01-Jul 29-Jun 25-Jul 01-Jul 29-Jun 01-Jul 28-Jun 30-Jun 30-Jun
12-Jul 18-Jul 08-Aug 05-Jul 01-Jul 30-Jun 23-Aug 29-Jun 12-Jul 05-Jul 08-Apr 25-Jul 13-Jul 28-Jun 05-Jul 07-Jul 27-Jul 10-Jul
SBT SBT/LUM SBT SSOP SSOP SSP/SBT SSP/SBT SBT LUM SBT/TGS SSP LUM SSP SSP/SBT/LUM RT-PCRSSP SBT
Kit manufacturer
Detection Method
One Lambda, Invitrogen One Lambda, Life Technologies One Lambda Immucor
Fluorescence Fluorescence Luminex Gel, Fluorescence
Yes
Immucor
Fluorescence Gel Fluorescence Fluorescence Fluorescence
Yes LUM LUM LUM
LUM SBT/LUM
Source of Primers and Probes Commercial In-house Other Kits Yes Yes SSOP Yes Yes Yes
LUM LUM
LUM SSOP
LUM LUM
LUM LUM LUM
LUM LUM
SBT LUM LUM SSOP
SBT/LUM LUM SSOP
LUM LUM SSOP
LUM
LUM
Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes
LUM LUM SBT/TGS
SSP/SBT LUM LUM SBT/TGS
Yes Yes Yes Yes
SSP SSP/LUM/NGS LUM SBT NGS LUM
Yes Yes Yes Yes Yes Yes Yes Yes Yes
Yes
One Lambda Immucor One Lambda One Lambda Invitrogen One Lambda, GenDx One Lambda, Conexio One Lambda, GenDx One Lambda One Lambda, GenDx Life Technologies Immucor One Lambda Olerup, Secore
Fluorescence Fluorescence Gel, Fluorescence Fluorescence Fluorescence Fluorescence Gel Gel Fluorescence Fluorescence Gel, Fluorescence
CTS, Biorad One Lambda, Olerup, GenDx One Lambda GenDx Olerup, Holotype GenDx, Immucor, Olerup Linkage Biosciences One Lambda, Olerup One Lambda, Olerup
Gel Gel, Fluorescence, Illumina MiSeq Fluorescence Gel, Fluorescence Gel, Illumina Miseq Gel, Fluorescence Fluorescence Gel, Fluorescence Gel, ABI3730
Yes Yes Yes Yes Yes Yes
Abbott, Atria, Olerup One Lambda, Olerup One Lambda Omixon One Lambda Lifecodes
Gel, Fluorescence Gel Gel Illumina Miseq Fluorescence Fluorescence
Yes Yes
Celera, Olerup BAG, Protrans
Gel, Big dye terminator Fluorescence, Mr SPOT
Yes Yes Yes Yes Yes Yes Yes Yes
Immucor One Lambda, Abbott, Olerup Protrans, Immucor One Lambda, Protrans One Lambda Innotrain One Lambda
Fluorescence Gel Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence
Yes SSP LUM
SSP
SSP
NGS RT-PCRSSP SSP
LUM SSOP
LUM LUM
SSP LUM
NGS
SSP
LUM
LUM SSOP/LUMSSOP/LUM SSP LUM
SSOP/LUM LUM SSP/SBT/LUM LUM LUM SSOP/LUM SSP LUM
Comments
DPB1
SBT SBT
LUM LUM
Yes
Yes Yes
UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
Third Generation Sequencing
NEQ-129 Issue 1 P 1 of 4 Effective Date 15/03/16
UK NEQAS for H&I Educational Scheme CLASS I DNA TYPING RESULTS DESPATCHED ON 28/06/2016 SAMPLE ED 04/2016 Lab No. 9 12 14 15 16 19 20 23 24 26 28 29 34 38 39 41 42 45 48 54 58 62 78 112 113 128 130 132 133 142 147 156 159 164 178 181 193 195 230 238 245 252 259 260 262 267 284 292 315 323
HLAA*
A*
11 11 11 11 11 11 11 11 11 11
24 24 24 24 24 24 24 24 24 24
B*
40 40:01 40:01/10/22N/43 40:01/55/141 40 40:01 40:01/216N/265N 40 40:01 40:01 40:01/14-308 11:01/12/19/88/178/224 24:02/07/19/50/145/260 40:01 11:01/02/03… 24:02/05/07… 40:01 11 24 40:01 11 24 40:01 NT NT NT 11 24 40:01 40:01 11 24 40:01 11 24 40:01 11 24 40 11:01:01:01 24:02:01:01 40:01:02 11 11:01:01:01 11 11 11:01 11:01 11
24 24:02:01:01 24 24 24:02 24:02 24
11
24
11 11 NT 11:01 11 11 11:01 11
24 24 NT 24:02 24 24 24:02 24
40 40:01:02 40 40:01:02 40:01 40:01 40:01 40:01 40:01/55/141/150/151/179/221/236/241/247/264/272/278/299/301 40:01 40:01 40:01 NT 40:01 40 40 40:01 40:01
11
24
40
11 11 11 11 11 11
24 24 24 24 24 24
40 40:01 40:01 40 40:01 40
B*
C*
40:10 40:10
04 04 04 04 04 04:03 04 04 04 04
40:10 40:10 40:10 40:10 40:10 40:10/282 40:10 40:10 40:10 40:10 NT 40:10 40:10:01 40:10 40:10 40 40:10:01:02
40:10:01 40:10:01 40:10 40:10 40:10 40:10:01 40:10:01 40:10 40:10 40:10 NT 40:10 40 40 40:10 40:10
04:03 04:03/171 04 04 NT 04
C*
Comments
04 04 04 04:01
04 04 04:01/09N/30/82 04:01/05/09N… 04 NT 04
04 04 04 04:03:01
04 04:82
04:03 04 04 04:03 04:03 04
04 04 04 04:82 04:82 04
04:03:01
40:01/226
04:03 04:03 NT 04:03 04 04 04:03 04
04 04:82 NT 04:82 04 04 04:82 04
Low number of technical personnel
04 40:10 40:10 40:10:01 40 40:10 40
04:03 04 04 04 04:01 04
04:01 04 04 04 04
UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-129 Issue 1 P 2 of 4 Effective Date 15/03/16
UK NEQAS for H&I Educational Scheme CLASS II DNA TYPING RESULTS DESPATCHED ON 28/06/2016 SAMPLE ED 04/2016 Lab No. 9 12 14 15 16 19 20 23 24 26 28 29 34 38 39 41 42 45 48 54 58 62 78 112 113 128 130 132 133 142 147 156 159 164 178 181 193 195 230 238 245 252 259 260 262 267 284 292 315 323
HLADRB1*
DRB1*
DRB3*
DRB4*
DQB1*
DQB1*
04 04 04 04 04 04 04 04:03 04 04
11 11 11 11 11 11 11 11:01 11 11
01/02/03 02 02 02 02 02 + 02 02
01/02/03 01 01 01 01 01 + 01 01
03 03 03:01 03:01/04/09 03 03 03 03:01 03 03
03 03 03;02 03:02/07/08
03 03:02 03 03
04:03 04:03/50/51… 04:03 04 NT 04
11:01 11:01/12/15… 11:01 11 NT 11
02 02 NT 02
01 01 NT 01
03:01 03:01/04/14… 03:01 03:01/04/14/27 NT 03
03:02 03:02/62/63… 03:02 02:01 03:02/62-64 NT NT 03 02
04 04 04 04:03:01
11 11 11 11:01:01
02/03 02 02
01 01 01
03 03 03 03:01:01:03
03 03 03:02:01
04 04:03:01 04:03 04 04:03 04:03 04
11 11:01:01 11:01 11 11:01 11:01 11
+
+
03 03:01:01 03 03 03:01 03:01 03
03 03:02:01 03:02 03 03:02 03:02 03
04:03:01
11:01:01
04 04 NT 04:03 04 04 04:03 04
11 11 NT 11:01 11 11 11:01 11
04
11
03
04 04 04 04 04 04
11 11 11 11 07 11
03 03 03 03 03:01 03
02
01
DPB1*
DPB1*
02:01
04:01
02:01
04:01
02:01
04:01
Comments
04:01 NT 04
02 02:01
04 04:01:01:01
02:01
04:01
02
04
NT 03:02 03 03 03:02 03
NT
NT
02
04
02:01
04:01
03 03 03 03 03:02 03
02:01
04:01
03
NT
02 02:02
NT
01 01:03
03 NT 03:01 03 03 03:01 03
02
04
02:01
04:01
Low number of technical personnel
UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-129 Issue 1 P 3 of 4 Effective Date 15/03/16
UK NEQAS for H&I Educational Scheme SEROLOGICAL TYPING RESULTS DESPATCHED ON 28/06/2016 SAMPLE ED 04/2016 Lab No.
HLAA
A
B
B
112 229
11 11
9 24
60 40
40
Bw4,w6
Cw
Cw
DR
DR
4
4
4 4
5 11
6
DR51, 52,53 52/53
DQ
DQ
7 3
8 3
U
Viability % T B
Comments
NOTE The tabulation includes all the information received. ? = doubtful assignment; wk = weak aintgen; NT = not tested "Not reported" is shown by a blank in the list
UK NEQAS for H+I is provided from the Welsh Blood Service, a division of Velindre NHS Trust © Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-129 Issue 1 P 4 of 4 Effective Date 15/03/16
