UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING...
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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A09/2014 DESPATCHED ON 16TH SEPTEMBER CONSENSUS HLA TYPE: HLA-A2,-; B13,B60; Cw3,-; DR16,-; DQ5,-
Lab No. 9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 294 297 299 315
Assessment HLA-A HLA-B HLA-Cw HLA-DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES
YES
YES
YES YES
HLA-DQ
HLA-A
YES YES YES YES YES YES YES YES YES
YES YES YES
YES YES YES YES YES YES YES
YES YES YES YES YES YES YES
YES YES YES YES
YES YES YES YES
YES YES
YES YES
YES
YES
YES YES
YES YES
YES
YES
2 2 2 2 2 2 2 2 2 2 2 2 2 2 203 2 2 2 2 2 2 2 NT 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
YES YES YES YES YES
YES YES YES YES
YES
YES YES
YES
A
2
2
NT 2
B
B
C
13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 NT 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13 13
60 60 (40) 60 60 60 60 60 60 60 60 60 60 60 60 (40) 60 60 (40) 60 60 60 60 60 60 NT 60 60 60 60 (40) 60 60 60 40 60 60 60 60 60 60 60 60 60 60 60
10
C
16 16 (2) 16 16 16 16 16 16 16
10
10 10
3
16 16 16 16 (2) 16 16 (2) 16
10 (3)
3 3
NT
DR
NT
16 16 16 16 NT 16 16
DR
DQ
16
5 5 (1) 5 5 5 5 5 5 5
16 (2)
NT 16
5 5 5 5 (1) 5 5 (1) 5 5 5 5 5 NT 5 5
16
5
16 16
5 5
16
5
16 16
5 5
16 16
5
3 3 3
© Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
DQ
5
5 (1)
NT 5
Date received
Date tested
17-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 16-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 18-Sep 17-Sep 17-Sep NT 17-Sep 17-Sep 17-Sep 18-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 17-Sep 17-Sep 16-Sep 19-Sep 17-Sep 17-Sep 17-Sep 17-Sep
18-Sep 19-Sep 17-Sep 18-Sep 17-Sep 17-Sep 18-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 19-Sep 19-Sep 19-Sep 18-Sep 18-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep NT 17-Sep 17-Sep 17-Sep 19-Sep 19-Sep 17-Sep 19-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 17-Sep 17-Sep 19-Sep 18-Sep 18-Sep 17-Sep 18-Sep
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A09/2014 DESPATCHED ON 16TH SEPTEMBER
Lab No. 9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 294 297 299 315
Supplemental DNA typing performed using: PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP/SSO PCR-SSP Luminex PCR-SSP PCR-SSP SBT
U
Viability % T B 95 90 90 99 90
90 99 95 99 95 95 100 90
PCR-SSO PCR-SSO PCR-SSO PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSP PCR-SSP PCR-SSP
100
PCR-SSO PCR-SSO
98 100
PCR-SSP PCR-SSP PCR-SSP
90 90 60 99 85 80 40 90 15
Tray Manufacturer One Lambda One Lambda One Lambda Innotrain Innotrain + One Lambda One Lambda One Lambda Innotrain + One Lambda One Lambda BioRad BioRad + One Lambda Inegen (One Lambda) One Lambda
90 100 Cell yield too low for typing 95
95
100
100
99 99
99 99 100
100 100 95 90
98 90
98 80
PCR-SSP PCR-SSP PCR-SSO PCR-SSP/SSO
98 100 100 80 100 95 100 98 100
PCR-SSP CDC PCR-SSP PCR-SSP PCR-SSO
Testing failures
90 95 80
95 100 80 90 98 Low B-cell viability 90
80
Comments
B locus ambiguity with PCR-SSP. Detected A26
Could not be tested serologically One Lambda BioRad + One Lambda BioRad + Innotrain BioTest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda BioRad BioRad One Lambda BioTest Ingen One Lambda One Lambda One Lambda BioRad + Innotrain One Lambda One Lambda One Lambda Ingen BioRad One Lambda
© Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
DRB performed by DNA typing because of poor recovery and low viability in B-cells, and to confirm single antigen assignment
