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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A10/2014 DESPATCHED ON 16TH SEPTEMBER CONSENSUS HLA TYPE: HLA-A11,A31; B35,B60; Cw4,Cw3; DR103,DR4; DQ5,DQ8
Lab No. 9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 294 297 299 315
Assessment HLA-A HLA-B HLA-Cw HLA-DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES
YES
YES
YES YES
HLA-DQ
HLA-A
A
B
B
C
C
DR
DR
DQ
DQ
Date received
Date tested
YES YES YES YES YES YES YES YES YES
YES YES YES
4
10
4
10
4
10
103 103 1 103 103 103 103 103 103
5 5 (1) 5 5 5 5 5 5 5
8 8 (3) 8 8 8 8 8 8 8
4
3
4
10 (3)
4 4
3 3
4 4 4 4 4 4 4
103 103 1 103 103 103 103
5 5 5 5 (1) 5 5 (1) 5
8 8 8 8 (3) 8 8 (3) 8
YES YES YES YES
4 4 4 4
103 103 1 1
5 5 5 5
8 8 8 8
17-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 16-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 18-Sep 17-Sep 17-Sep
18-Sep 19-Sep 17-Sep 18-Sep 17-Sep 17-Sep 18-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 19-Sep 19-Sep 19-Sep 18-Sep 18-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep
YES YES
YES YES
4 4
103 103
5 5
8 8
YES
YES
4
01:03
5
03:02
YES YES
YES YES
4 4
1 103
5 5
8 8
YES
YES
60 60 (40) 60 60 60 60 60 60 60 60 60 60 60 60 (40) 40 60 (40) 60 60 60 60 60 60 NT 60 60 60 60 (40) 60 60 60 40 60 60 60 60 60 60 60 60 60 60 60
4 4 4 4 4 4 4 4 4
YES YES YES YES
35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 NT 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35
10
YES YES YES YES YES YES YES
31 31 (19) 31 31 31 31 31 31 31 31 31 31 31 31 31 31 (19) 31 31 31 31 31 31 NT 31 31 31 31 (19) 31 31 31 31 31 31 31 31 31 31 31 31 31 31 31
4
YES YES YES YES YES YES YES
11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 NT 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11
4
103
5
8
4 4
103 103
5 5
8 8
4 4
103 103
5
8
17-Sep 17-Sep 17-Sep 18-Sep 18-Sep 17-Sep 18-Sep 17-Sep 17-Sep 17-Sep 17-Sep 17-Sep 17-Sep 16-Sep 19-Sep 17-Sep 17-Sep 17-Sep 17-Sep
17-Sep 17-Sep 17-Sep 19-Sep 19-Sep 17-Sep 19-Sep 17-Sep 18-Sep 17-Sep 18-Sep 18-Sep 17-Sep 17-Sep 19-Sep 18-Sep 18-Sep 17-Sep 18-Sep
YES YES YES YES YES
YES YES YES YES
YES
YES YES
YES
4
3
4 4
3 3
© Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A10/2014 DESPATCHED ON 16TH SEPTEMBER
Lab No.
Supplemental DNA typing performed using:
9 11 20 25 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 164 181 187 193 194 206 223 225 227 232
PCR-SSO PCR-SSP PCR-SSP PCR-SSP/SSO PCR-SSO PCR-SSP PCR-SSP Luminex
238
PCR-SSP
245 262 268 279 284 285 289 294 297 299 315
PCR-SSO PCR-SSP SBT
U
Viability % T B
90
95 90 95 99 95 90 99
90
99 95
99
95
Testing failures
95 100
80
80
100
100
99 99
99 99
90 PCR-SSO PCR-SSO PCR-SSO PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSO PCR-SSP PCR-SSP PCR-SSP
Cell viability too low for typing
100 98 100
100 100 100 100
98 100 100 80 100 95 100 98 100
PCR-SSP CDC 90 95 >80
One Lambda BioRad + One Lambda BioRad + Innotrain BioTest One Lambda One Lambda One Lambda One Lambda
98 90
One Lambda One Lambda One Lambda BioRad BioRad One Lambda BioTest
95
Ingen
98 80
PCR-SSO PCR-SSP/SSO
PCR-SSO
100
100 80 95 98 90 60 >80
Comments
One Lambda One Lambda One Lambda Innotrain Innotrain + One Lambda One Lambda One Lambda Innotrain + One Lambda One Lambda BioRad BioRad + One Lambda Inegen (One Lambda) One Lambda
90 99 85 90 95
100
Tray Manufacturer
One Lambda One Lambda One Lambda BioRad + Innotrain One Lambda One Lambda One Lambda Ingen BioRad One Lambda
© Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
Could not be tested serologically Very low expression of HLA-B35 by serological typing
As the B35 was difficult to identify by serology we performed B genetic kit to confirm and B35 kit which identifies a very rare B*35:41 which is found difficult by serology and associated DRB1*01:03. Rare allele B*35 only detected by PCR-SSO, negative on serological trays
