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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A10/2016 DESPATCHED ON 20TH SEPTEMBER 2016 CONSENSUS HLA TYPE: HLA-A2, A-; B7, B57; Cw7, Cw-; DR7, DR103; DQ5, DQ9
9 11 20 28 54 58 62 101 119 120 123 133
A YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
315 YES YES 323 YES YES
YES YES
Lab. No.
HLADQ YES YES YES YES YES YES YES YES YES YES YES
YES YES
YES YES YES YES YES YES YES YES YES YES YES YES
A 2 2 2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
2 2
A
2 2
2 2
2
2 2 2
2
B 7 7 7 7 7 7 7 7 7 7 7 7
B 57 57(17) 57 57 57 57 57 57 57 57 57 57
7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
57 57(17) 57 57 57 57 57 57 57 57 57 57 57(17) 57 57 57 57 57 57 57 57 57 57 57 57 57 57
7 7
57 57
C 7
C
7
7
7
7
7
7 7
7
DR 7 7 7 7 7 7 7 7 7 7 7 7
DR 103 103 1 103 103 103 103 103 103 103 103 103
DQ 5 5(1) 5 5 5 5 5 5 5 5 5 5(1)
DQ 9 9(3) 9 9 9 9 9 9 9 9 9 9(3)
Date received 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 22-Sep
7 7 7
103 103 103
5 5(1) 5
9 9(3) 9
7 7 7 7 7 7 NT
103 103 103 103 103 103 NT
5 5 5 5 5 5 NT
9 9 9 9 9 9 NT
7
103
5
9
7
103
5
9
7
103
5
9
7 7 7
103 103 103
1 1 5
3 3 9
7
103
23-Sep 22-Sep 21-Sep 22-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 22-Sep 22-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 21-Sep 22-Sep 23-Sep 21-Sep 20-Sep 22-Sep
7
103
5
9
7 7 7
7
22-Sep 22-Sep
Viability % Date tested U T B Comments 22-Sep 90 90 22-Sep 95 95 Cw not typed by serology 21-Sep 90 90 21-Sep 90 85 22-Sep 95 90 21-Sep 95 90 21-Sep 99 95 22-Sep 100 100 22-Sep 90 60 22-Sep 100 100 28-Sep 90 22-Sep 94 1) Molecular typing confirms different allels for C*07 2) The homozygosity for -A2 must be confirmed by family results 23-Sep 90 23-Sep 22-Sep 100 100 23-Sep 21-Sep 90 90 22-Sep 99 99 99 21-Sep 90 90 22-Sep 97 21-Sep 100 100 21-Sep 99 99 21-Sep 100 21-Sep 100 23-Sep 99 23-Sep 95 21-Sep 98 21-Sep 98 95 22-Sep 100 21-Sep 100 22-Sep 95 95 23-Sep 100 21-Sep 99 95 22-Sep 100 22-Sep 98 98 90 30 23-Sep 22-Sep 100 100 22-Sep 80 22-Sep 95 95 1) Locus A tested also by a different typing trays to confirm SA assignment 2) DRB performed by DNA typing to confirm DR103 assignment 22-Sep >90 >90 22-Sep 85
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING SCHEME 1A - HLA PHENTOYPING - METHODOLOGY 2016 Lab. No. 9 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
Source of HLA Class I Typing trays One Lambda One Lambda Own Tray Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda Innotrain One Lambda One Lambda One Lambda One Lambda One Lambda Biorad, Immucor Biotest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda One Lambda Biotest, Innotrian One Lambda Biorad, Innotrain One Lambda One Lambda One Lambda Biorad, BAG One Lambda One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda One Lambda Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda Immucor One Lambda One Lambda One Lambda One Lambda Biorad One Lambda Invitrogen One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda
Suplemental DNA typing performed PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO, Luminex PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSO RT-PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP PCR-SSO PCR-SSO PCR-SSP PCR-SSP/SSO
One Lambda
PCR-SSO PCR-SSO
One Lambda
PCR-SSP
One Lambda One Lambda One Lambda
PCR-SSP PCR-SSO
Biorad, BAG One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSO
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 2 of 2
