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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A01/2018 DESPATCHED ON 20TH MARCH 2018 CONSENSUS HLA TYPE: HLA-A25, A29; B18, B44; Cw12, Cw16; DR7, DR13; DQ2, DQ6
Lab No. 11 20 28 54 58 62 101 119 120 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 286 289 292 294 299 315 323
A YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR DQ YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES
YES YES
YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES
HLAA
A
B
B
25(10) 25 25 25 25 25 25 25 25 25(10) 25 25(10) 25 25 25 25 25 25 25 25 25 25 25(10) 25 25 25 25 25 25 25 25 NT 25 25 25 25 25 25
29(19) 29 29 29 29 29 29 29 29 29(19) 29 29(19) 29 29 29 29 29 29 29 29 29 29 29(19) 29 29 29 29 29 29 29 29 NT 29 29 29 29 29 29
18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 NT 18 18 18 18 18 18
44(12) 44 44 44 44 44 44 44 44 44(12) 44 44(12) 44 44 44 44 44 44 44 44 44 44 44(12) 44 44 44 44 44 44 44 44 NT 44 44 44 44 44 44
C
C
12
16
12
16
12
16
12 12
16 16
12
16
NT
NT
12
16
DR
DR
DQ
DQ
Date received
Date tested
7 7 7 7 7 7 7 7 7 7 7
13(6) 13 13 13 13 13 13 13 13 13(6) 13
2 2 2 2 2 2 2 2 2 2 2
6(1) 6 6 6 6 6 6 6 6 6(1) 6
7
13
2
6
7 7 7 7 7 7
13 13 13 13 13 13
2 2 2 2 2 2
6 6 6 6 6 6
21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 22-Mar 20-Mar 21-Mar 21-Mar 22-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 04-Apr 22-Mar 22-Mar 22-Mar 21-Mar 21-Mar 22-Mar
22-Mar 22-Mar 21-Mar 22-Mar 21-Mar 21-Mar 22-Mar 22-Mar 22-Mar 23-Mar 23-Mar 22-Mar 22-Mar 23-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 21-Mar 22-Mar 22-Mar 23-Mar 22-Mar 21-Mar 21-Mar 28-Mar 21-Mar 22-Mar 22-Mar 22-Mar
7
13
2
6
7
13
2
6
7 NT 7 7 7 7 7
13 NT 13 13 13 13 13
2 NT 2 2 2
6 NT 6 6 6
22-Mar 23-Mar 23-Mar 21-Mar 21-Mar 22-Mar
U
Viability % T B 90 90 95 95 95 99
95 100
Comments
80 90 95 95 90 95 90 100
95 75
60 Aggregated cells 100
100
95 99 90 100 99
95 99 90 100 99
98 98
Not enough cells to perform HLA class II by CDC 100 95 90
98 90 100 100 92
90 >80 95
98 70
60 Aggregated cells
98 90
98 80
92 Delayed delivery 98 60 90 50 DR confirmed by DNA typing because of high mortality in B-cells 80
80
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A01/2018 DESPATCHED ON 20TH MARCH 2018 CONSENSUS HLA TYPE: HLA-A25, A29; B18, B44; Cw12, Cw16; DR7, DR13; DQ2, DQ6
Lab No. 11 20 28 54 58 62 101 119 120 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 286 289 292 294 299 315 323
Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO PCR-SSP
Source of HLA Class I Typing trays One Lambda In-house Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda One Lambda
PCR-SSP, Luminex PCR-SSP Luminex PCR-SSO PCR-SSOP PCR-SSP PCR-SSP/SSO PCR-SSP
Innotrain One Lambda Innotrain One Lambda One Lambda Innotrain, Immucor Biotest One Lambda
Innotrain, One Lambda One Lambda
PCR-SSO Luminex PCR-SSP/SSO PCR-SSP
One Lambda One Lambda One Lambda One Lambda
One Lambda One Lambda One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSP
One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda
PCR-SSO
One Lambda
One Lambda
PCR-SSP Luminex
BAG, One Lambda One Lambda
BAG, One Lambda One Lambda
Comments
One Lambda One Lambda Innotrain One Lambda
One Lambda
DNA typing performed only when an antigen can not be assessed because of criteria for antigen assignment or technical reasons (eg. low cell viability)
- Denotes 2017 methodology/No methodology received
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 2 of 2
