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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A07/2018 DESPATCHED ON 20TH NOVEMBER 2018 CONSENSUS HLA TYPE: HLA-A2, A3; B44, B65; Cw5, Cw8; DR13, DR-; DQ6, DQ-
Lab No. 11 20 28 54 58 62 119 120 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323 401
A YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR DQ YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES
YES YES
YES YES YES YES YES YES YES YES YES
YES YES YES YES
YES YES YES
HLAA
A
B
B
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 NT
3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 NT
44(12) 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 NT
65(14) 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 65 NT
C
C
5
8
DR
DR
13(6)
5
8
5
8
5 5
8 8
13 13 13 13 13 13
5 NT
DQ
6(1) 13
6 6 6 6 6 6
13
6
13
6
13 13 13 13 13 13
5
DQ
13
13
6 6 6 6 6 6
13
6
13
6
13 13 13 13 13 13
6
6
6
6
8
8 NT
NT
13 13 NT
6 6 6 NT
6 6 NT
Viability % T B
Date received
Date tested
U
21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 22-Nov 26-Nov 21-Nov 21-Nov 21-Nov 21-Nov 22-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 22-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 21-Nov 23-Nov 26-Nov 21-Nov 21-Nov 21-Nov 22-Nov NT
23-Nov 21-Nov 21-Nov 22-Nov 21-Nov 21-Nov 22-Nov 22-Nov 23-Nov 27-Nov 22-Nov 22-Nov 22-Nov 21-Nov 22-Nov 22-Nov 22-Nov 21-Nov 21-Nov 21-Nov 22-Nov 22-Nov 23-Nov 21-Nov 22-Nov 22-Nov 21-Nov 22-Nov 24-Nov 21-Nov 23-Nov 27-Nov 21-Nov 21-Nov 22-Nov 22-Nov NT
98 95 95 90 95 100 90
Comments
90 50 80 90 90 100
90 80
80
100
100 Supplemental DNA typing
95 99 85
95 99 85 100 99 99
100 99 99 90 90 95 95
Split of B14 - B65 found by PCR-SSP
98 98 100 100 80 0.75 98 40
98 Poor quality cells 99 98
80 98
100 80
80 50 50 B-cell viability was poor
NT
NT Technical issue
70 90 NT
60
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A07/2018 DESPATCHED ON 20TH NOVEMBER 2018 CONSENSUS HLA TYPE: HLA-A2, A3; B44, B65; Cw5, Cw8; DR13, DR-; DQ6, DQ-
Lab No. 11 20 28 54 58 62 119 120 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323 401
Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO PCR-SSP PCR-SSP, Luminex PCR-SSP Luminex PCR-SSO PCR-SSOP PCR-SSP PCR-SSP/SSO PCR-SSP
Source of HLA Class I Typing trays One Lambda In-house Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda Innotrain One Lambda Innotrain One Lambda One Lambda Innotrain, Immucor Biotest One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda One Lambda Innotrain, One Lambda One Lambda
RT-PCR Luminex PCR-SSP/SSO PCR-SSOP
One Lambda One Lambda One Lambda One Lambda
One Lambda One Lambda One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSP
One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda
PCR-SSO
One Lambda
One Lambda
PCR-SSP PCR-SSP
BAG, One Lambda One Lambda
BAG, One Lambda One Lambda
One Lambda
One Lambda
Comments
One Lambda One Lambda Innotrain One Lambda
One Lambda
- Denotes 2017 methodology/No methodology received
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 2 of 2
