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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A01/2016 DESPATCHED ON 02ND FEBRUARY 2016 CONSENSUS HLA TYPE: HLA-A2, A23; B18, B50; Cw6, Cw7; DR1, DR11; DQ5, DQ7
Lab. No. 9 11 20 28 41 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
A YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
HLADQ YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES
YES YES
YES YES
YES
A 2 2 NT 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A 23 23(9) NT 23 23 23 23 23 23 23 23 23 23 23 23(9) 23 23 23 23 23 23 23 23 23 23 23(9) 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23
B 18 18 NT 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18
B 50 50(21) NT 50 50 50 50 50 50 50 50 50 50 50 50(21) 50 50 50 50 50 50 50 50 50 50 50(21) 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50
C 6
C 7
NT
NT
6
7
6
7
6
7
6
7
6 6
7 7
6
7
6 6
7 7
6 6
7 7
DR 1 1 NT 1 1 1 1 1 1 1 1 1 1 1 1 1
DR 11 11(5) NT 11 11 11 11 11 11 11 11 11 11 11 11 11
DQ 5 5(1) NT 5 5 5 5 5 1 5 5 5 5(1) 5 5(1) 5
DQ 7 7(3) NT 7 7 7 7 7 7 7 7 7 7(3) 7 7(3) 7
1 1 1 1 1 1 1
11 11 11 11 11 11 11
5 5 5 5 5 5 5
7 7 7 7 7 7 7
1
11
5
7
1
11
5
7
1
11
5
7
1 1 1 1 1 1
11 11 11 11 11 11
5 5 5
5 5
7 7 7
7 7
Date received 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 04-Feb
Date tested
04-Feb 05-Feb 03-Feb 03-Feb 03-Feb 04-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 04-Feb 03-Feb 03-Feb 03-Feb 03-Feb 03-Feb 04-Feb 04-Feb 04-Feb 03-Feb 03-Feb 02-Feb 05-Feb
04-Feb 05-Feb 04-Feb 04-Feb 04-Feb 08-Feb 04-Feb 03-Feb 03-Feb 03-Feb 03-Feb 04-Feb 04-Feb 04-Feb 04-Feb 03-Feb 04-Feb 04-Feb 03-Feb 04-Feb 08-Feb 03-Feb 03-Feb 04-Feb 05-Feb 04-Feb 04-Feb 03-Feb 03-Feb 05-Feb
04-Feb 04-Feb 03-Feb 11-Feb 04-Feb 03-Feb 03-Feb 03-Feb 04-Feb 04-Feb
U
Viability % T 95 90
B 95 60
Comments
Technical failure
>90 95 100 100 95 100 90 94 85
99
94 85
85
100
100
90 99 98
90 90 98 100 90 99 90
100 80 99 100
>90 90 75 100 95 100
99 100 90 95
98 95 100
95
95 100 95
95
98 100
98 15 90
80
80 80
90
85
100 15 90 95 80
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING SCHEME 1A - HLA PHENTOYPING - METHODOLOGY 2016 Lab. No. 9 11 20 28 41 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
Source of HLA Class I Typing trays One Lambda One Lambda Own Tray Innotrain, One Lambda MC Diagnostics One Lambda Innotrain, One Lambda One Lambda One Lambda Biorad One Lambda One Lambda One Lambda One Lambda One Lambda Biorad, Immucor Biotest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda One Lambda Biotest, Innotrian One Lambda Biorad, Innotrain Formedic, One Lambda One Lambda One Lambda Biorad, BAG One Lambda One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda One Lambda Innotrain, One Lambda MC Diagnostics One Lambda Innotrain, One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Biorad One Lambda Invitrogen One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda
Suplemental DNA typing performed PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSO, Luminex PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSO RT-PCR-SSP PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP/SSO PCR-SSO PCR-SSO PCR-SSP PCR-SSP PCR-SSP PCR-SSP/SSO
One Lambda
PCR-SSO PCR-SSP
One Lambda
PCR-SSP
Formedic, One Lambda One Lambda One Lambda
PCR-SSP PCR-SSO
Biorad, BAG One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSO
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 2 of 2
