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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A06/2016 DESPATCHED ON 07TH JUNE 2016 CONSENSUS HLA TYPE: HLA-A2, A3; B7, B35; Cw4, Cw7; DR11, DR15; DQ6, DQ7
Lab. No. 9 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
A YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
Assessment HLAB Cw DR YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
DQ YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES
YES YES
YES YES
YES
A 2 NT 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A 3 NT 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3
B 7 NT 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
B 35 NT 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 71
C 4 NT 4
HLAC 7 NT 7
4
7
4
7
4
7
4 4
7 7
DR 11 NT 11 11 11 11 11 11 11 11 11 11(5) 11 11(5) 11
DR 15 NT 15 15 15 15 15 15 15 15 15 15(2) 15 15(2) 15
DQ 6 NT 6 6 6 6 6 6 6 6 6 6(1) 6 6(1) 6
DQ 7 NT 7 7 7 7 7 7 7 7 7 7(3) 7 7(3) 7
11 11 11 11:01 11 11 11
15 15 15 15 15 15 15
6 6 6 06:02 6 6 6
7 7 7 03:01 7 7 7
11
4
7
4 4
7 7
4
7
15
6
7
11
15
6
7
11
15
6
7
11 11 11
15 15 15
11 11
15 15
1 6 6
6
7 7 7
7
Date received Date tested 08-Jun 08-Jun 09-Jun 10-Jun 08-Jun 08-Jun 08-Jun 08-Jun 08-Jun 09-Jun 08-Jun 08-Jun 09-Jun 09-Jun 08-Jun 09-Jun 09-Jun 09-Jun 09-Jun 09-Jun 10-Jun 10-Jun 10-Jun 10-Jun 10-Jun 09-Jun 10-Jun 09-Jun 10-Jun 10-Jun 13-Jun 08-Jun 09-Jun 09-Jun 10-Jun 09-Jun 09-Jun 08-Jun 08-Jun 08-Jun 09-Jun 09-Jun 09-Jun 09-Jun 09-Jun 10-Jun 08-Jun 13-Jun 09-Jun 10-Jun 08-Jun 08-Jun 09-Jun 09-Jun 08-Jun 08-Jun 09-Jun 09-Jun 09-Jun 09-Jun 10-Jun 11-Jun 09-Jun 09-Jun 10-Jun 10-Jun 09-Jun 09-Jun 09-Jun 10-Jun 08-Jun 09-Jun 08-Jun 09-Jun 09-Jun 09-Jun 09-Jun 09-Jun 09-Jun 09-Jun
Viability % U T B Comments 95 95 Insufficient material to perform confirmational PCR 90 80 90 95 90 99 95 99 99 100 100 80 60 100 100 90 30 30 80 80 80 99
100 100 95 95 98
75 80 98
99
99 98
100 98 98 100 98 100 98 95
95 100
75 98 100 90
95 90
100 98
50 100 98 80 80
98 30 100 100
80
95 80
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING SCHEME 1A - HLA PHENTOYPING - METHODOLOGY 2016 Lab. No. 9 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 285 289 292 294 297 299 315 323
Source of HLA Class I Typing trays One Lambda One Lambda Own Tray Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda Innotrain One Lambda One Lambda One Lambda One Lambda One Lambda Biorad, Immucor Biotest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda One Lambda Biotest, Innotrian One Lambda Biorad, Innotrain One Lambda One Lambda One Lambda Biorad, BAG One Lambda One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda One Lambda Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda One Lambda Immucor One Lambda One Lambda One Lambda One Lambda Biorad One Lambda Invitrogen One Lambda One Lambda One Lambda One Lambda Ingen, One Lambda
Suplemental DNA typing performed PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO, Luminex PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSO RT-PCR-SSP PCR-SSP PCR-SSP/SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP/SSO PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSP PCR-SSP/SSO
One Lambda
PCR-SSO PCR-SSP/SSO
One Lambda
PCR-SSP
One Lambda One Lambda One Lambda
PCR-SSP PCR-SSO
Biorad, BAG One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSO
UK NEQAS for H+I is operated by Velindre NHS Trust, UKAS proficiency testing provider 8351 © Copyright Notice: Confidential report; no data may be published without permission from UK NEQAS for H+I
NEQ-114, iss 1 Effective Date 14/03/16 P. 2 of 2
