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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A08/2017 DESPATCHED ON 18TH JULY 2017 CONSENSUS HLA TYPE: HLA-A1, A2; B44, B65; Cw5, Cw7; DR4, DR7; DQ2, DQ7 Assessment HLAB Cw DR DQ
A
A
B
B
C
C
DR
DR
DQ
DQ
Date received
Date tested
U
11 YES YES YES YES 20 YES YES YES YES YES
1 1
2 2
44(12) 44
65(14) 65
5 5
7 7
4 4
7 7
2 2
7(3) 7
17-Jul 19-Jul
18-Jul 19-Jul
90
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
44 44 44 44 44 44 44 44 44(12) 44 44(12) 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44 44
65 65 65 65 65 65 65 65 65(14) 65 65(14) 65 65 65 65 65 65 65 65 65 65 65 65 14 65 65 65 65 65 65 65 65 65 65 65 14
4 4 4 4 4 4 4 4 4 4 4 4
7 7 7 7 7 7 7 7 7 7 7 7
2 2 2 2 2 2 2 2 2 2 2 2
7 7 7 7 7 7 7 7 7(3) 7 7(3) 7
4 4 4 4 4 4
7 7 7 7 7 7
2 2 2 2 2 2
7 7 7 7 7 7
19-Jul 19-Jul 19-Jul 18-Jul 19-Jul 20-Jul 20-Jul 20-Jul 21-Jul 20-Jul 19-Jul 20-Jul 21-Jul 19-Jul 19-Jul 19-Jul 19-Jul 19-Jul 20-Jul 19-Jul 20-Jul 20-Jul 20-Jul 19-Jul 19-Jul 19-Jul 20-Jul 18-Jul 19-Jul 19-Jul 20-Jul 21-Jul 19-Jul 20-Jul 20-Jul 21-Jul
19-Jul 20-Jul 19-Jul 19-Jul 26-Jul 21-Jul 20-Jul 24-Jul 21-Jul 21-Jul 20-Jul 21-Jul 23-Jul 19-Jul 19-Jul 20-Jul 19-Jul 19-Jul 20-Jul 20-Jul 21-Jul 21-Jul 21-Jul 19-Jul 19-Jul 19-Jul 20-Jul 19-Jul 24-Jul 19-Jul 20-Jul 24-Jul 20-Jul 20-Jul 24-Jul 21-Jul
Lab No.
28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323
A
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES
YES YES YES YES YES YES YES YES YES
YES YES YES YES
HLA-
5
7
5
7
5
7
5 5
5
5
7 7
4
7
2
7
4
7
2
7
4 4 4 4 4 4
7 7 7 7 7 7
2
7
2 2
7 7
7
7
2
7
Viability % T B 90
90 Class II serological testing was not performed on this samples due to the on going issues with the class II dynabeads. Please note supplementary DNA typing was performed to obtain class II type using PCR-SSP
99 95 95 99 90 99 90 85 90
Comments
90 90 50 90 70 99
85 90
90
100
80
99 80
90 80 100 99 98
95
100 99 98 100 95 95 98 98 100 100 96
85
92 98 98
70 100 90 >80
95 98 40 90 70 >80
90
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A08/2017 DESPATCHED ON 18TH JULY 2017 CONSENSUS HLA TYPE: HLA-A1, A2; B44, B65; Cw5, Cw7; DR4, DR7; DQ2, DQ7
Lab No. 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323
Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSO
Source of HLA Class I Typing trays One Lambda In-house Innotrain, One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda Innotrain, One Lambda
PCR-SSO
Innotrain, One Lambda
One Lambda
Luminex PCR-SSP
Immucor, One Lambda One Lambda One Lambda
Luminex PCR-SSO PCR-SSOP PCR-SSP PCR-SSP, SSO PCR-SSP
Innotrain One Lambda One Lambda Innotrain One Lambda One Lambda Innotrain, Lifecodes Biotest One Lambda
RT-PCR Luminex PCR-SSP, SSO PCR-SSP
One Lambda One Lambda One Lambda One Lambda
One Lambda One Lambda One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSP
One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda
PCR-SSO
One Lambda
One Lambda
PCR-SSP PCR-SSP
BAG One Lambda One Lambda
BAG One Lambda
Comments
One Lambda One Lambda Biorad One Lambda
One Lambda
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 2 of 2
