UK NEQAS FOR H&I SCHEME 1A - HLA TYPING...
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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A10/2017 DESPATCHED ON 03rd October 2017 CONSENSUS HLA TYPE: HLA-A2, A3; B7, B51; Cw7, Cw14; DR11, DR13; DQ6, DQ7 Assessment HLAB Cw DR DQ
A
A
B
B
C
11 YES YES NO YES YES 20 YES YES YES YES YES
2 2
3 3
7 7
51(5) 51
7
2 2
3 3
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3
7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
51 51 51 51 51 51 51 51 51(5) 51 51(5) 51 51 51 51 51 51 51 51 51 51 51 51 51 51 51 51 51 51 51
2 2 2 2 2
3 3 3 3 3
7 7 7 7 7
51 51 51 51 51
Lab No.
28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323
A
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
NO NO YES NO NO YES NO NO NO NO NO YES YES NO NO NO NO NO NO NO NO NO NO NO NO NO NO NO YES NO NO NO NO NO NO NO
YES YES YES YES YES YES YES YES NO YES YES YES NO YES YES YES YES YES YES NO NO NO NO NO YES NO NO YES NO YES YES YES YES YES YES NO
YES YES YES YES YES YES YES YES NO YES YES YES NO YES YES YES YES YES YES NO NO NO NO NO YES NO NO YES NO YES NO YES YES NO YES NO
HLAC
7
14
7 7
14 14
7 7
7 7
14 14
DR
DQ
DQ
Date received
Date tested
11(5) NT
13(6) NT
6(1) NT
7(3) NT
03-Oct 04-Oct
04-Oct 06-Oct
90 90
70 60 Unable to obtain class II HLA type by serological methods for sample 1A10 due to poor viability of cells and a low cells count meaning there was insufficient material to repeat the testing.
11 11 11 11 11 11 11 11 11 11 11(5) 11
13 13 13 13 13 13 13 13 13(6) 13 13(6) 13
6 6 6 6 6 6 NT 6 6(1) 6 6(1) 6
7 7 7 7 7 7 NT 7 7(3) 7 7(3) 7
04-Oct
04-Oct
04-Oct 04-Oct 04-Oct 04-Oct 04-Oct 04-Oct 06-Oct
04-Oct 04-Oct 04-Oct 05-Oct 05-Oct 05-Oct 06-Oct
95 90 95 99
95 >90 80 90
11 11 11 11 11 11
11
11 7
7
13 13 13 13 13 13
13
13
6 6 6 NT 6 1
6
6
7 7 7 7 7 7
7
11
13
6
7
11 11 11 11
13 13 13 13
6 6
7 7
6
05-Oct
04-Oct 04-Oct 04-Oct 03-Oct 04-Oct
05-Oct 04-Oct 05-Oct 04-Oct 05-Oct
04-Oct 04-Oct 04-Oct 04-Oct 04-Oct 04-Oct
04-Oct 05-Oct 04-Oct 16-Oct 05-Oct 04-Oct
04-Oct 03-Oct 05-Oct 06-Oct 04-Oct
04-Oct 04-Oct 05-Oct 12-Oct 05-Oct
06-Oct 04-Oct 04-Oct 06-Oct 05-Oct
06-Oct 04-Oct 04-Oct 10-Oct 05-Oct
100 90 85 90 90
7
Comments
70 85 Splits not done for DQ 90 90
90
Molecular typing confirmed result for HLA-C locus as 7,14
100
100
99 99 90
99 99 80
99
99 99
96 99 100 100 98
7
14
14
05-Oct
U
Viability % T B
DR
98 100 100 95
80
95 100 98
100 95 90
85
99 80 90
75
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 1 of 2
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A10/2017 DESPATCHED ON 03rd October 2017 CONSENSUS HLA TYPE: HLA-A2, A3; B7, B51; Cw7, Cw14; DR11, DR13; DQ6, DQ7
Lab No. 11 20 28 54 58 62 101 119 120 123 133 139 142 145 149 154 159 163 181 186 193 194 206 223 225 227 238 245 262 268 279 284 289 292 294 299 315 323
Supplemental DNA typing performed using: PCR-SSP PCR-SSP PCR-SSO
Source of HLA Class I Typing trays One Lambda In-house Innotrain, One Lambda
Source of HLA Class II Typing trays One Lambda One Lambda Innotrain, One Lambda
PCR-SSO
Innotrain, One Lambda
One Lambda
Luminex PCR-SSP
Immucor, One Lambda One Lambda One Lambda
Luminex PCR-SSO PCR-SSOP PCR-SSP PCR-SSP, SSO PCR-SSP
Innotrain One Lambda One Lambda Innotrain One Lambda One Lambda Innotrain, Lifecodes Biotest One Lambda
PCR-SSO Luminex PCR-SSP, SSO PCR-SSP
One Lambda One Lambda One Lambda One Lambda
One Lambda One Lambda One Lambda One Lambda
PCR-SSP PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSP
One Lambda Innotrain Innotrain One Lambda One Lambda One Lambda
PCR-SSO
One Lambda
One Lambda
PCR-SSP PCR-SSP
BAG One Lambda One Lambda
BAG One Lambda
Comments
One Lambda One Lambda Biorad One Lambda
One Lambda
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-114 Issue 2 Effective Date 10/10/16 P. 2 of 2
