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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B07&08/2018 DESPATCHED ON 16 OCTOBER 2018 Summary of Results Total tested (excl. Equivocal) Positive Negative NT/Equivocal % Positive % Negative Consensus
Lab No
1 3 4 7 8 9 10 11 12 14 15 17 19 20 21 22 23 24 25 26 28 29 31 32 34 35 37 39 40 41 42 45 46 47 48 54 55 57 58 62 65 66 67 72 76 77 78 83 84 85 86 87 90 96 101 103 104 106 107 108 109 110 111 112 113 115 126 127 128 129 130 133 137 142 150 153 154 156 159 174 176 185 198 209 219 223 224 225 232 234 236 245 252 255 256 257 269 273 276 278 279 288 292 295 300 305 306 307 315 317 319 324 327 334 339 340 348 349 353 357 359 362 365 368 372 373 377 378 402 404 407 409
Donor HLA type
1B07/2018
A2, A11; B27, B62; Cw2, Cw7; DR4, DR12; DQ7, DQ8
Donor HLA type
1B08/2018
A2, A24; B27, B44; Cw2, Cw5; DR4, DR12; DQ7, DQ8
128
129
128 0 4 100.0% 0.0%
129 0 3 100.0% 0.0%
Positive
Positive
Result 1B07/2018 Positive
Result 1B08/2018 Positive
Date received 17-Oct
Date tested 24-Oct
Positive
Positive
18-Oct
18-Oct
Positive
Positive
17-Oct
29-Oct
Positive
Positive
17-Oct
18/2018
Serological
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
18-Oct
19-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
18-Oct
25-Oct
Molecular
Positive
Positive
17-Oct
22-Oct
Molecular
Positive
Positive
17-Oct
22-Oct
Molecular
Positive
Positive
17-Oct
19-Oct
Serological and Molecular
Positive
Positive
17-Oct
19-Oct
Molecular
Positive
Positive
19-Oct
Positive
Positive
18-Oct 18-Oct
19-Oct
Serological
Positive
Positive
17-Oct
19-Oct
Molecular
Positive
Positive
18-Oct
22-Oct
Molecular
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
22-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Positive
Positive
17-Oct
25-Oct
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
PCR-SSP Primers and Probes
Method
1B08/2018
B*27- only defined to first field
B*27- only defined to first field
B*27:03/B*27:05 string
B*27:03/B*27:05 string
Own design
Fluorescence
B*27:03/27:05:02:01/27:10/27:17
B*27:03/27:05:02:01/27:17
B*27:03/27:05/27:05Q/27:13
B*27:03/27:05/27:05Q/27:13
B27:05/47/90/116-162
B27:05/47/90/116-162
Molecular Molecular
17-Oct
19-Oct
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
18-Oct
19-Oct
Serological
Equivocal
19-Oct
23-Oct
Serological
Positive
Positive
18-Oct
19-Oct
Positive
Positive
17-Oct
23-Oct
Positive
Positive
24-Oct
30-Oct
Molecular
Positive
Positive
17-Oct
19-Oct
Serological
Positive
Comments
1B07/2018
Molecular
Equivocal
Positive
High Resolution Typing HLA-B*27 alleles Detection system
Molecular Serological
Own design
Gel
Molecular
Molecular
Commercial
Gel
Molecular
Positive
17-Oct
19-Oct
Positive
Positive
17-Oct
17-Oct
Molecular
Equivocal
Equivocal
25-Oct
26-Oct
Serological
Molecular
Positive
Positive
18-Oct
22-Oct
Molecular
Positive
Positive
19-Oct
19-Oct
Serological
Positive
Positive
22-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
16-Oct
23-Oct
Positive
Positive
18-Oct
18-Oct
Serological
Positive
Positive
17-Oct
17-Oct
Serolgical and Molecular
Positive
Positive
10-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Positive
Positive
18-Oct
24-Oct
Molecular
Positive
Positive
17-Oct
22-Oct
Molecular
Positive
Positive
18-Oct
18-Oct
Serological
Positive
Positive
19-Oct
19-Oct
Serological
Positive
Positive
18-Oct
19-Oct
Serological
Positive
Positive
22-Oct
29-Oct
Molecular
Positive
Positive
18-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
19-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological and Molecular
Positive
Positive
18-Oct
20-Oct
Serological
Results are in the grey zone so need to be referred for confirmation by PCR
Molecular
NT
NT
NT
NT
Positive
Positive
17-Oct
19-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
23-Oct
29-Oct
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
30-Oct
Serological and Molecular
Positive
Positive
19-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
19-Oct
Serological and Molecular
Positive
Positive
18-Oct
18-Oct
Serological and Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
19-Oct
26-Oct
Molecular
Positive
Positive
18-Oct
25-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
26-Oct
Molecular
Positive
Positive
19-Oct
23-Oct
Molecular
Positive
Positive
19-Oct
19-Oct
Positive
Positive
20-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
16-Oct
22-Oct
Molecular
Positive
Positive
31-Oct
02-Nov
Molecular
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
25-Oct
29-Oct
Molecular
Positive
Positive
22-Oct
23-Oct
Positive
Positive
23-Oct
30-Oct
Molecular
Positive
Positive
18-Oct
22-Oct
Serological
Positive
Positive
17-Oct
18-Oct
Positive
Positive
18-Oct
22-Oct
Positive
Positive
17-Oct
25-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
24-Oct
25-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Positive
Positive
17-Oct
22-Oct
Molecular
Positive
Positive
17-Oct
22-Oct
Serological
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
18-Oct
19-Oct
Serological
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
30-Oct
31-Oct
Molecular
Positive
Positive
15-Oct
30-Oct
Positive
Positive
17-Oct
25-Oct
Positive
Positive
17-Oct
20-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
22-Oct
25-Oct
Molecular
Positive
Positive
17-Oct
23-Oct
Molecular
Positive
Positive
18-Oct
19-Oct
Serological
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
23-Oct
23-Oct
Serological
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
Exon 2 and Exon 3 positive for both
Exon 2 and Exon 3 positive for both
Positive in Exon 2
Late delivery of samples
Molecular
Own design
Fluorescence
Commercial
Fluorescence
Commercial
Fluorescence
For both samples potential alleles B*27:03/27:05
For both samples potential alleles B*27:03/27:05
All HLA-B*27 subgroups
All HLA-B*27 subgroups
HLA- B*27 Exon 2 and Exon 3 in both samples
HLA- B*27 Exon 2 and Exon 3 in both samples
We are not using HR molecular typing method
Molecular
Molecular
Molecular Molecular
Molecular
ReadyGene B27 InnoTrain
Molecular
18-Oct
Serological
Equivocal
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
18-Oct
18-Oct
Serological
Positive
Positive
17-Oct
23-Oct
Positive
Positive
17-Oct
24-Oct
Molecular
Positive
Positive
17-Oct
18-Oct
Serological
Positive
Positive
17-Oct
18-Oct
Molecular
Positive
Positive
18-Oct
19-Oct
Serological
Positive
Positive
19-Oct
19-Oct
Molecular
Positive
Positive
19-Oct
19-Oct
Serological
Positive
Positive
05-Nov
05-Nov
Positive
Positive
18-Oct
18-Oct
Molecular
Positive
Positive
17-Oct
17-Oct
Serological
Positive
Positive
24-Oct
24-Oct
Serological
Positive
Positive
22-Oct
23-Oct
Serological
Commercial
Gel
Commercial
Gel
Molecular
RIDA®GENE HLA-B27 #11228
Serological
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
NEQ-115 Issue 2 P. 1 of 2 Effective Date 10/10/16
UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING
HLA-B27 TYPING RESULTS OF SAMPLES 1B07&08/2018 DESPATCHED ON 16 OCTOBER 2018 Lab No 20 46
Source of pos/neg control cells used (if applicable) In-house Commercial- Pos QC - CRISP HLA B27 pos cells
Designation Anti-B27-FITC/CD3-PE CD3, PE, BD
Monoclonal 1 Specificity B27+B7
Manufacturer BD
Designation Anti-B27 B27 Anti-HLA B27 , FITC, BD
Beckman Coulter Beckman Coulter Immunotech Beckman-Coulter BD
ABCm3 and FD705 (PE) Anti-B7 PE FD705 HLA-B7 PE HLA-B27
Monoclonal 2 Specificity
Manufacturer One Lambda
Designation
Monoclonal 3 Specificity
Manufacturer
67 128 137 324 377
In-house
UCHT1 (PE-Dyomics649) Anti-B27 FITC GLA-ABCm3/BB7.1 HLA-B27 FITC CD3 PE
CD3 HLA-B27/B7 HLA B27 FITC 100%
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
HLA-B27 HLA-B27 HLA-B27 PE 92%
Beckman Coulter Beckman Coulter One Lambda Beckman-Coulter BD
BB7.1 (unlabelled) CD45 PerCP-Cy5.5
HLA-B27
Beckman Coulter BD
NEQ-115 Issue 2 P. 2 of 2 Effective Date 10/10/16
